山东胶东地区规模鸡场三种主要病毒感染情况的调查

Investigation on Infection of Major Three Viral Diseases by PCR in Large-Scale Chicken Farms of Jiao-dong District in Shandong Province

为了解2009下半年山东胶东地区规模化鸡场主要疫病的感染情况,应用RT-PCR技术对山东青岛、潍坊和烟台三地区送检的774份具有呼吸道症状的病鸡病料进行了新城疫病毒(NDV)、传染性支气管炎病毒(IBV)和H9亚型禽流感病毒(AIV)3种主要病毒的感染情况调查。调查结果显示,三个地区3种病毒的感染率为25.58%,NDV、IBV和AIV(H9)的阳性率分别为8.14%、11.37%和6.07%。青岛地区NDV、IBV的阳性率均最高,分别为12.47%、14.55%。2种病毒混合感染的阳性率达2.84%,以IBV+NDV二重混合感染临床最为常见。调查结果表明,山东省胶东地区NDV、IBV和AIV(H9)的感染情况比较普遍,且存在一定程度的混合感染,为有效预防和控制山东省规模鸡场该3种主要疫病提供了科学依据。

In order to investigate the infection of major viral diseases in large-scale chicken farms of jiao-dong district in Shandong Province in the latter half of 2009,the investigation on infection of NDV,IBV and AIV(H9) was performed by established RT-PCR technique.774 suspicious samples collected from large-scale chicken farms in Shandong Province(Weifang,Qingdao and Yantai district) were detected.The results showed that the positive rates of these three viruses was up to 25.58% totally in three districts and the positive rates of NDV,IBV and AIV(H9) were 8.14%,11.37% and 6.07%,respectively.In three districts the positive rates of NDV(12.47%) and IBV(14.55%) were both highest in Qingdao.Chickens co-infected with dual viruses accounted for 2.84% from the detected samples.The infection of IBV+NDV was most serious in all co-infectioned samples.The results demonstrated that NDV,IBV and AIV(H9) were very prevalent in the commercial chickens in Jiao-dong District,offering the scientific evidence to prevent and control these diseases in Shandong Province.