唐山地区致鸡肝炎大肠埃希菌分离鉴定及药物筛选试验

Escherichia coli Isolation and Identification of Chicken Liver from Tangshan Region and Drug Screening

从唐山地区无菌采集临床上疑似大肠埃希菌病鸡的肝脏,进行细菌分离培养,根据细菌培养特性观察、形态学检查和生化试验及动物试验结果,鉴定出致鸡肝炎的大肠埃希菌18株。将分离的18株致病性大肠埃希菌对12种药物进行了药敏试验,结果表明分离的大肠埃希菌对大多数氨基糖苷类高度敏感,其中对硫酸新霉素的敏感率最高达100%;对青霉素类耐药程度较高,甚至对先锋V具有较严重的抗药性,其高敏感率接近0%;对喹诺酮类抗生素、黄连素也具有不同程度的耐药性。联合用药试验结果表明硫酸新霉素和头孢拉定联合用药对大肠埃希菌的抑制作用特别明显。临床试验证明硫酸新霉素和头孢拉定联合用药对大肠埃希菌预防保护率高达94%,治疗率在84%以上。

clinically aseptic collection of suspected E.coli in chicken liver from Tangshan region,isolated and cultured for bacteria,according to observed characteristics of bacterial culture,morphological examination and biochemical tests and the results of animal experiments,identified the large intestine caused by hepatitis chicken 18 sp.18 will be separated from Escherichia coli of the 12 kinds of drug sensitivity test,results showed that the majority of the separation of E.coli is highly sensitive to aminoglycosides,neomycin sulfate in which the highest sensitivity of 85.7%;on higher levels of penicillin-resistant,and even a Pioneer V.There are more serious resistance,its high sensitivity rate of close to 0%;they also have vary degrees of resistance to quinolone antibiotics and berberine.Proven in clinical trials and neomycin sulfate cefradine combined prevention of E.coli up to 94% protection rate,treatment rate of more than 84%.