低渗诱导栉孔扇贝三倍体及与其它方法的比较

Triploid Induction in Chlamys farreri by Hypotonic Treatment and The Comparison with Other Treatment Methods

采用低渗抑制受精卵第2极体(PB2)的释放诱导栉孔扇贝(Chlamys farreri)三倍体。水温20℃时,分别进行不同盐度处理(8~20)、不同起始处理时间(受精后15~40 min)和不同持续处理时间(10~25 min)的实验,对三倍体处理组和对照组幼虫的生长进行观察,同时将低渗、6-DMAP、热休克、冷休克4种诱导方法进行对比。结果表明:受精后30 min开始处理20 min,诱导率最高,可达(92.36±2.41)%,卵裂率为(49.78±6.51)%,孵化率为(61.82±1.63)%;D形幼虫期,处理组幼虫表现出明显的生长优势(P【0.01),不仅生长速度快,而且发育到变态时个体比二倍体大;低渗诱导的三倍体率与6-DMAP相比无显著差异(P】0.05);与热休克和冷休克相比差异显著(P【0.01)。

Hypotonic treatment was used in triploid induction in Chlamys farreri by inhibiting polar body Ⅱ(PB2) releasing.Different hypotonic treatments,including salinity(8~20),initial treating time(15~40 min after fertilization),and duration time(10~25 min),were tested at 20 ℃ water temperature.Triploid group and diploid group were designed to imestigate the growth of larva.Four different triploid induction methods were compared including hypotonicity,6-DMAP,cold shock and heat shock.The Results indicated that the optimal hypotonic treatment was 30 min after fertilization,which can resulted in the highest triploidy rate which was up to(92.36±2.41)%,(49.78±6.51)% cleavage rate and(61.82±1.63)% hatchery rate.The triploid group showed obvious growing advantage in the larvae stage(P<0.01),the growing speed of triploid larvae was rapid,and the shell length was longer than diploid larvae when it growed to metamorphosis.Comparison of triploidy rate showed no significant difference between the treatment of hypotonicity and 6-DMAP,while there was significant difference between the treatment of hypotonicity and temperature shock.