摘要
为了探讨甲状腺激素受体在大菱鲆(Scophthalmus maximus)体内的表达情况及在细胞水平三碘甲状腺原氨酸(T3)对甲状腺激素受体(TR)表达量的影响,利用荧光定量PCR法检测了大菱鲆8个组织中甲状腺激素受体TRαA、TRβ的表达量,并设计了0,50,75,100和200 nmol/L 5个不同浓度的T3处理大菱鲆肾细胞(SMKC),采用实时荧光定量PCR法测定T3处理后甲状腺激素受体TRαA、TRβ的表达量。结果表明,甲状腺激素受体TRαA、TRβ在大菱鲆不同组织中的表达量各不相同,但两者均在肾脏组织中表达量最高。在不同浓度T3处理后甲状腺激素受体的表达量存在明显的差异,在低浓度条件下,TRαA、TRβmRNA的表达量增加,当T3浓度达到100 nmol/L时,TRαA、TRβmRNA表达量明显降低,说明50,75 nmol/L的T3能够引起细胞中甲状腺激素受体TRαA、TRβ表达量的增加,而100,200 nmol/L的T3会相对抑制细胞中甲状腺激素受体TRαA、TRβ的表达量。
In order to study the expression of thyroid hormone receptor(TR) gene in Scophthatmus maximus and the effects of triiodothyronine(T3) on the expression of thyroid hormone receptor(TR) gene at the cellular level,Real-time fluorescent quantitative PCR was applied to detect the expression of TRαA mRNA and TRβ mRNA in eight different tissues of Scophthatmus maximus and the SMKC that was treated by T3 in five different concentrations(0,50,75,100 and 200 nmol/L).The results showed that the expression of TRαA mRNA and TRβ mRNA were various in the different tissues of Scophthatmus maximus.Both TRαA mRNA and TRβ mRNA showed their highest expression in the kidney tissue.There was a significant difference on the expression of thyroid hormone receptor(TR) after treated by T3 using different concentrations.The expression of TRαA and TRβ mRNA were increased in low concentration.However,the TRαA and TRβ mRNA expression decreased significantly when the concentration of T3 up to 100nM.It was shown that 50nM and 75nM of T3 can increase the TRαA and TRβ mRNA expression,while 100nM and 200nM of T3 can inhibit their expression relatively.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第S1期105-109,共5页
Periodical of Ocean University of China
基金
国家自然科学基金项目(30571420)
国家教育部博士项目基金(20040264001)
上海市重点学科水生生物学建设项目(S3070)资助
