乙型慢性活动性肝炎患者外周血淋巴细胞亚群白细胞介素-2受体的初探

Interleukin-2 Receptor Expression on Human Lymphocyte Subsets in Patients With Chronic Active Hepatitis B

应用间接免疫荧光法,放射免疫分析法,微量细胞培养法及斑点杂交技术,检测了27例乙型慢性活动性肝炎(CAH-B)患者及7例正常对照者CD4-、CD8-及B细胞膜上白细胞介素-2受体(mIL-2R,CD26抗原为其β链),各亚群细胞培养上清及病人同期血清中可溶性白细胞介素-2受体(sIL-2R),CD4-细胞培养上清中IL-2活性。结果CAH-B病人外周血,CD25抗原阳性的CD4+、CD8+细胞及B细胞较正常对照者减少,且CD25抗原阳性的CD4+、CD8+细胞减少较B细胞明显;CD4+细胞培养上清中IL-2活性较正常人降低,并与病情的轻重、CD4-细胞mIL-2R的表达呈正相关;CAH-B病人血清sIL-2R增高,但培养上清sIL-2R与正常对照无显著性差异。结果提示CAH-B病人外周血CD4+、CD8+及B细胞mIL-2R表达均减弱;CAH-B病人体内T细胞免疫应答功能受损可能较B细胞受损明显;CAH-B病人体内IL-2产生水平低下与病情轻重有某种关联;CAH-B患者血清中增高的sIL-2R可能来自肝内浸润的免疫活性细胞mIL-2Rβ链脱落。

Objective The technigue of rosete formation,which involved a serius of monoclonal antibodies labled with the sheep red blood cell,was emplpyed to divide the periphery blood mononuclear cell(PBMC)into CD4+、CD8+、Bsubsers in 27 parients with CAH-B.The CD25 antigen(mIL-2Rβ)posilive cells in these cellular subsels were identified by indirect immunolluorescence assay.The level of IL-2 in CD4-cell supernatants was dereted by microcellular culture assay,and the soluble interleukin-2 receptor(sIL-2R)in smpernstants by radioimmunosssay.The results showed that the populstions of CD25 positive cells in CAH-B patients were lower than that in healthy controlsi and the decrease of CD25 positive cells in CD4+ and CD8+ cells was inore significant compared whth that in B cells.The level of IL-2 in CD4+ cell supernalants decreased in CAH-B patients,was correlated positively with CD4+、CD8+ and B cells after culure bad no significant difference between the group of CAH-B patierls and that of healthy controls.The serum level of sIL-2R increased in chese patienls.From our sudies,it is comcludod that IL-2R expression on CD4+、CD8+ and H cells decreases in CAH-B patients;in which,the impairmcpt of immure response in T cells serus puch more severe than in B cellss.The reduction of IL-2 production from activaled CD4+ cell is related to the severity of liver impairments and expression of IL-2R on CD4+ cell.There is no obvioos coordination between the levels of sIL-2R in serum and supernatant.Therefore,the elevated serum sIL-2R may be attributed to the increased relcase from the β cham of mIL-2R on the iniiltrated lymphocytes in liver.