系统性红斑狼疮患者Th1、Th2细胞因子表达异常与T细胞亚群功能失调相关性研究

The study of relativity between abnormal expression of cytokine Th1、Th2 which in systimic lupus erythematiosus patients and dysfunction of T cell subpopulation

目的探讨系统性红斑狼疮(SLE)患者Th1、Th2细胞因子表达异常与T细胞亚群功能失调的相关性。方法用流式细胞仪测定病例组与对照组T细胞表面标志CD3、CD4、CD8的表达情况。用酶联免疫吸附测定法测定病例组与对照组血清IFN-γ及IL-4水平。结果SLE患者CD3+CD4+细胞较正常对照组明显降低(P<0.01),CD4+CD8+细胞较正常对照组明显增加(P<0.01),CD4+/CD8+T细胞比例倒置;血清IFN-γ水平较正常对照组明显增高(P<0.01),而血清IL-4水平较正常对照组明显降低(P<0.05)。结论SLE患者体内细胞免疫功能紊乱,以Th1细胞亢进为主,产生大量Th1细胞因子,介导免疫调节反应。同时,CD8+T细胞功能亢进,CTL细胞参与机体组织损伤,进一步导致免疫紊乱及免疫病理损伤加重。

Objective To explore the relativity between abnormal expression of cytokine Th1 and Th2 which in systimic lupus erythematiosus patients and dysfunction of T cell subpopulation.Methods The expression of CD3、 CD4 and CD8 on the T cell surface in experiment group and control group were determined by flow cytometry.The lever of TFN-γ and IL-4 in serum of experiment group and control group were determined by enzy-linked immunosorbent assay.Results CD3+CD4+ cell of SLE patients decreased dramatically compared with that of control group(p<0.01),CD4+CD8+ cell of SLE patients increased dramatically compared with that of control group(p<0.01),CD4+/CD8+ cell ratio reversed;The lever of TFN-γ in SLE patients serum increased dramatically compared with that in control group(p<0.01),The lever of IL-4 decreased obviously(p<0.05).Conclusion The cell immune in SLE patients become dysfunction,in which hyperfunction of Th1 cell are major,a lot of Th1 cytokine produced and lead to immune regulate reaction.At the same time,CD8+ cell become hyperfunction,CTL cell partake in organism tissue injury,immune disorder and immune pathological injury aggravated.