Twist在大肠癌中的表达及其与临床病理指标的关系

The Expression of Twist gene in Colorectal Carcinomals and relation with clinical pathological marker

目的探讨肿瘤相关基因Twist表达在大肠癌临床病理诊断中的应用价值。方法应用鼠抗人Twist单克隆抗体采用S-P免疫组织化学方法检测112例不同类型大肠癌组织及40例正常肠粘膜组织中Twist的表达并与临床病理指标比较。结果在112例大肠癌组织中,Twist基因阳性表达78例;在正常组织中其阳性表达为15例,两者比较具有显著统计学差异(χ2=11.21,p=0.001);进一步综合临床病理资料统计分析,Twist在大肠癌组织中表达与肿瘤的分化程度、肿瘤浸润、淋巴结转移、Duke’s分期有关(p值分别为0.008、0.017、0.044、0.011),均有显著差异;而与病人年龄、性别无关(p值为0.286、0.887)。结论Twist基因表达检测能较好的判断大肠癌肿瘤分化程度、浸润、淋巴结转移等,在大肠癌诊断中作用明确,对临床病理学诊断有明确的指导作用。

Objective To investigate the expression of Twist in colorectal carcinomas and identify the clinical and pathological use of it.Methods The expressions of Twist were detected by Immunohistochemical S-P method in 112 cases of colorectal carcinomas and 40 cases of normal colorectal tissues from surgical resections.Results The positive rate of Twist in colorectal carcinomas was 69.6% while the positive rate in normal gastric tissues was 17.5%,a significant difference was found between them(χ2=12.11,p=0.001); Twist overexpression was related with the tumor differentiation(χ2=9.575,p=0.008),the depth of invasion(χ2=8.183,p=0.017),lymph node metastasis(χ2=4.05,p=0.044) and Duke's staging(χ2=11.114,P=0.011),while not related with age(p=0.286),sex(p=0.887) difference.Conclusion Twist may play an important role in the formation and development of colorectal carcinomas,The expression of Twist is a useful marker in the clinical and pathological diagnosis of colorectal carcinomas.