酶法和多层膜干片法测血清脂肪酶的方法学比较

Comparison of delerninging serum lipase by enzymatic method and Multi-layer dry slides method

目的了解酶法和多层膜干片法测定血清脂肪酶结果的偏倚大小和实验特点。方法分别用酶法和多层膜干片法测定血清脂肪酶,对两种方法进行比较。结果酶法和多层膜干片法的线性范围分别为0～500U/L和0～2000/UL;批内重复性实验的平均变异数分别为2.12%和1.28%;批间重复性实验的平均变异系数为3.23%和1.64%;平均回收率分别为101.1%和103.6%;相关性试验的回归方程为Y=0.43X+5.65,r为0.986;加入胆红素<342.08umol/L。血红蛋白<5g/L,甘油三脂<10.08mmol/L对两种方法无显著干扰;参考值范围分别为0～60U/L和0～300U/L。结论酶法和多层膜干片法适合临床应用,有很好的相关性,也有一定的差异性。

Objective To evahlate the relative bias between serum lipase results determined by enzymatic method and multi-layer dry slides method.Methods Serum lipase was determined by enzyamatic method and multi-layer dry alides method.Results The linear ranges were 0-500U/L and 0-2000/UL respectively,average within-run CVs were 2.12% and 1.28% respectively; average between-run CVs were 3.23%and 1.64% tespectively;the average recovery rates were 101.1% and 103.6%;The regressive formular of earrelative exporiment was Y=0.43X±5.65,r=0.986.Adding Bilirubin<342.08umol/L,Hemoglobin<5g/L, Triglyeeride<10.08mmol/L,had no significantly interference on determing.The referece ranges of two methods were 0-60U/L and 0-200U/L respectively.Conclusion Enzymatic method and multi-layer dry slides method can be used in clinical,they have good correlativity,and somewhat difference.