新生仔猪腹泻的体外抑菌治疗的研究

新生仔猪腹泻是由肠毒素性大肠杆菌(ETEC)产生一种或数种菌毛粘附素粘附于小肠黏膜上皮细胞及结膜层上而引起的。在实验中给禁食初乳的新生仔猪感染K88、K99、987P菌毛型的ETEC而导致腹泻,然后口服复方中药免疫球蛋白生物制剂进行治疗。体外抑菌研究结果表明:在大肠杆菌中加入复方中药免疫球蛋白生物制剂后,在最初1～3小时内其抑菌作用最强,3小时以后,其作用急剧降低。到5小时,起抑菌作用消失。体外粘附试验结果表明:效价为1:2560复方中药免疫球蛋白生物制剂在体外可以有效的抑制大肠杆菌在小肠上皮细胞的粘附,而且ETEC在小肠不同部位的菌毛受体并无明显的特异性。

Diarreal disease caused by enterotoxi- genic Escherichia coli(ETEC)is by far the most common enteric colibacillosis encountered in neonatal piglets.the orally administered the pro- duction of compound Chinese herbal medicine immunoglobulins in a dose-dependent fashion against infection with each of the three homolo- gous strains of E.coli in passive immunization trails with a colostrums-deprived piglet model of enterotoxigenic E.coli diarrhea.The study of bacterostasis in vitro,adhesion assay in vitro,the lever of cell immunity and antibody. In vitro,the production of compound Chinese herbal medicine immunoglobulins block the growth of enter enteropaphogenic strains from 0 to 3 hours,whereas after 3 hours,the function of it was become wane,after 5 hours,the effect was not observed.In adhesion assay,the E.coli strains adhered equally to pocine duodenal and ileal ep- ithelial cells but failed to do so in the produc- tion of compound Chinese herbal medicine im- munoglobulins with higher titer.