肺癌患者热应激蛋白70诱导表达能力的研究

The study on induced expression of heat-shock protein(HSP)70s in lung carcinoma patients

目的分析肺癌患者外周血淋巴细胞热应激蛋白70(HSP70)亚型HSP72、HSP73基础表达与热损伤条件下表达水平,探讨HSP70在肺癌发生、发展中的意义。方法选取肺癌患者和与之年龄、性别、职业史、居住地等相近的对照人群,采取分离淋巴细胞分别于37℃和42℃进行培养和热损伤处理,用流式细胞仪测定细胞中HSP72和HSP73表达水平。结果对照组HSP72、HSP73基础表达水平(荧光强度)分别为21.97±2.40和12.77±0.66,比肺癌组(19.0±2.12和11.74±0.68)显著升高(P<0.01);热处理后对照组HSP72表达水平(25.06±6.37)比基础水平显著升高(P<0.01),肺癌组(19.55±1.76)则与基础水平差异无统计学意义(P>0.05);热处理后HSP73均未出现明显改变。结论肺癌患者HSP70基础表达低下,HSP72诱导能力低下,可能与肺癌的易感性有关。

Objective To analyze the expression level of HSP72 and HSP73,the subtypes of HSP70, in peripheral blood lymphocytes from lung carcinoma patients on both basic and heat injury conditions,and to explore the significance of HSP70 in the development of lung cancer.Methods Lung cancer patients were selected'as experimental group,and the health people with similar age,gender,vocational history and inhabi- tation to the experimental group were chosen as control group.The blood lymphocytes from both groups were isolated,cultured and treated with heat injury at either 37℃or 42℃.The expressions of HSP72 and HSP73 in the isolated lymphocytes were determined by Flow Cytometry.Results There were much higher expressions of HSP72 and HSP73 in control group(21.97±2.40 vs 12.77±0.66)than which in experimental group(HSP72 19.0±2.12 vs HSP73 11.74±0.68,P<0.01).Treatment with heat injury did significantly increase the expres- sion of HSP72 in control group(25.06±6.37)than that in basic group(P<0.01),whereas there was no differ- ence in HSP72 expression between lung cancer group(19.55±1.76)and basic group.The expression of HSP 73 remains unchangeable after treatment with heat injury between any groups.Conclusion There was a low ba- sic expression of HSP 70 and a low inducing ability of HSP 72 in patients suffered from lung cancer,which may contribute to the increased vulnerability to lung cancer.