COX-2、Ki-67和VEGF在膀胱移行细胞癌中的表达及意义

Expression of COX-2,Ki-67 and VEGF in bladder transitional epithelial cancers and their significance

目的探讨环氧化酶-2(COX-2)在膀胱移行细胞癌中的表达及临床意义。方法应用免疫组织化学法检测50份膀胱移行细胞癌患者手术后存档标本和10份正常膀胱黏膜组织中COX-2、血管内皮生长因子(VEGF)和增生细胞核抗原(Ki-67)的表达。结果COX-2在膀胱移行细胞癌组织中阳性表达率为80%,而在正常膀胱黏膜组织中无表达,差异有统计学意义。而且不同分期、不同分级间表达差异亦有统计学意义,即与分级、分期呈正相关;初发组COX-2表达高于复发组;单发组与多发组差异无统计学意义。在膀胱移行细胞癌组织中COX-2的表达与Ki-67的表达呈正相关;COX-2与VEGF在膀胱移行细胞癌组织中的表达呈正相关。结论COX-2在肿瘤发生、发展中可能起着促进细胞增生的作用。VEGF的表达与COX-2呈正相关,表明VEGF可能是COX-2促进肿瘤血管生成的重要介质。

Objective To study COX-2 expression in bladder transitional cell carcinoma in order to explore the relationship between the immunohistochemical staining and biological behavior of bladder cancer. Methods The expression of COX-2,Ki-67,and VEGF was examined in 50 cases of bladder transitional cell carcinoma and 10 normal bladder mucosa by means of immunohistochemical technique.Results The expression of COX-2 in BTCCs was higher than that in normal bladder mucosa.The positive rate of COX-2 was 80% in BTCCs while it was not detected in normal bladder mucosa.COX-2 was highly positively related to the grade,stage and prognosis of tumors.The expression of COX-2 in poorly differentiated,invasive or re- curring BTCCs was higher than that in well differentiated superficial or nonrecurring ones.But there was not significant difference between the solitary tumour and multiple ones.There were positive correlation both be- tween COX-2 and Ki-67 and between COX-2 and VEGF.Conclusion The high expression of COX-2 may benefit increase in bladder cancer cell proliferative activity.VEGF expression in COX-2 positive tumor was significantly higher than that in COX-2 negative tumor.