摘要
目的探讨淋巴管内皮透明质酸受体-1(LYVE-1)mRNA在早期子宫内膜癌组织及癌旁组织中的表达及其意义。方法采用反转录-聚合酶链反应(RT-PCR)方法对22例Ⅰ、Ⅱ期子宫内膜癌组织(T组)及其癌旁组织(PT组)、13例正常子宫内膜组织(NE组)中LYVE-1的表达进行检测。结果LYVE-1在三组均有表达,PT组表达最高(1.2440±0.1036),NE组和T组表达依次降低(分别为0.9889±0.1365,0.7812±0.1796),三组相对含量比较差异有统计学意义(P<0.01)。PT组Ⅰ期和Ⅱ期患者LYVE-1表达差异有统计学意义(P<0.05)。而PT组LYVE-1表达水平与子宫内膜癌临床病理分级、病理类型和肌层浸润程度无相关性(P>0.05)。结论LYVE-1在癌旁组织高表达,提示早期子宫内膜癌癌旁组织有新生淋巴管生成,从而可能进一步促进淋巴结转移。
Objective To study the expression and clinical significance of lymphatic vessel endothe- lial hyaluronan receptor LYVE-1 in early stage endometrial carcinoma and its peritumoral stroma.Methods Expression of LYVE-1 mRNA were assessed by semi-quantitative reverse transcription-polymerase chain re- action(RT-PCR)in 22 cases of early stage endometrial carcinoma(T group),its corresponding peritumoral stro- ma(PT group),and 13 cases of normal endometrium(NE group).Results LYVE-1 mRNA were detected in all 3 groups.The expression level of LYVE-1 in PT group was the highest(1.2440±0.1036)compared with the other 2 groups.LYVE-1 mRNA in NE group and T group were 0.9889±0.1365 and 0.7812±0.1796 respec- tively.There was a significant difference among the 3 gronps(P<0.01).In PT group,the LYVE-1 mRNA was higher in stageⅡthan in stageⅠ(P<0.05),while the expression of LYVE-1 mRNA were irrelevant to the surgico-pathological grade,pathological subtype and myometrial infiltration depth(P>0.05).Conclusion The high expression of LYVE-1 in peritumoral stroma indicates lymphangiogenesis,and then may promote lym- phatic metastasis.
出处
《肿瘤研究与临床》
CAS
2007年第3期159-161,共3页
Cancer Research and Clinic
