粪便中K-ras基因突变检测在大肠癌诊断中的意义

Significance of detecting the mutations of K-ras gene in faecal for diagnosis colorectal carcinoma

目的探讨K-ras基因突变在大肠癌诊断中的意义。方法该院2005年5月至2006年7月住院患者经肠镜确诊的36例大肠癌患者和24例大肠良性疾病患者,24例正常健康体检患者对照组。通过改良酚-氯仿抽提法提取患者粪便中DNA,PCR-RFLP方法检测K-ras基因第一外显子12密码子的突变。结果大肠癌患者粪便中K-ras突变率为77.8%,大肠良性疾病者为25.0%,正常对照组为8.3%。结论检测粪便中K-ras基因可以提高大肠癌的诊断。

Objective To study the significance of detecting the mutations of K-ras gene in faecal for diagnosis colorectal carcinoma.Methods 36 cases suffering from colorectal carcinoma,24 cases from colorectal benign diseases and 24 cases from healthy subjects,all cases had been diagnosed by enteroscope. K-ras gene mutation at the codon 12 was detected by PCR-RFLP,DNA was taken from faecal in modified method.Results K-ras mutation rate in colorectal carcinoma was 77.8 %,colorectal benign diseases was 25.0 %,healthy cases was 8.3 %.Conclusion Detecting the mutations of K-ras gene in faecalmay improve the rate of diagnosing colorectal carcinoma.