摘要
以异丙醇为介质,用环氧丙烷与碱化壳聚糖反应合成水溶性羟丙基壳聚糖,并用FTIR,1H-NMR和XRD对其结构进行表征,元素分析法测定其取代度;以MTT法研究该衍生物对兔角膜基质细胞生长的影响。结构分析结果表明:在壳聚糖的C6—OH上成功引入了羟丙基基团;MTT实验结果表明:该衍生物对角膜基质细胞的生长无抑制作用,具有良好的细胞相容性,为其在组织工程化角膜中的应用提供了一定的理论基础。
In this study,a water-soluble chitosan derivative hydroxypropyl chitosan(HPCTS) was synthesized by chitosan(CTS) and propylene oxide under alkali conditions.It was characterized by Fourier-transform infrared(FTIR),1H-NMR and X-Ray diffraction(XRD).The substitution degree of HPCTS was measured by Elemental analysis.The effect of HPCTS on growth of rabbit corneal stromal cells was also evaluated by using MTT assay.The structure characterization results showed that hydroxypropyl group was successfully grafted on the chitosan chain;MTT testing suggested that HPCTS had no obvious cytotoxicity,and had good cytocompatibility,which offers a theoretical basis for its application in tissue engineered cornea.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第S2期131-134,共4页
Periodical of Ocean University of China
基金
国家高技术研究发展规划项目(2006AA02A132
2007AA091603)资助
关键词
羟丙基壳聚糖
制备
表征
角膜基质细胞
细胞相容性
hydroxypropyl chitosan
preparation
characterization
corneal stromal cells
cytocompatibility
