从异养小球藻USTB-01中提取纯化叶黄素研究

Extraction and purification of lutein from Chlorella USTB-01

在成功筛选异养小球藻种USTB-01并完成高效发酵培养的基础上,主要针对从小球藻干粉中提取纯化叶黄素进行了研究。得到的优化工艺是:采用正己烷-乙醇混合试剂(体积比6.7∶1.0)从藻细胞中提取叶黄素1 h,固液质量比为1∶50,可以从小球藻粉中高效提取出叶黄素和叶黄素酯类。用质量分数20%的NaOH-水溶液,在50℃下皂化6 h,皂化剂用量为1∶40,可以高效地将叶黄素酯转化为叶黄素。通过条件优化实验,可将藻粉中游离叶黄素的含量由0.09%提高到0.32%,获得叶黄素的纯度达到了80.6%,为进一步将异养小球藻作为叶黄素来源的产业化生产奠定了重要的研究基础。

Based on the isolation and fermentational culture of a heterotrophic Chlorella USTB-01,the extraction of lutein and the saponification of lutein esters from Chlorella USTB-01 were investigated.It showed that the mixture of henxane-ethanol(V/V,6.7∶1.0) was an efficient solvent to extract lutein from the microalgal cells for 1 h at the ratio of 1∶50(g/mL) between solid and liquid,at room temperature.The optimal saponification parameters were that 20 wt% NaOH-water was used as saponification liquid at the ratio of 1∶40(g/mL) under 50℃ for 6 h.Under these conditions,most lutein esters were converted to free lutein.The content of free lutein could increase from 0.09% to 3.20% in Chlorella cells after the saponification and the purity of 80.6% lutein was obtained,which laid a very important foundation of study for the production of lutein from Chlorella USTB-01.