乳头瘤病毒与女阴疣状增生性疾病以及宫颈鳞状细胞癌关系的研究

Studies on Relationships of Papilloma Virus of Proliferative Verrucous Vaginal Diseases and Squamous Carcinoma of Cervix

本文用ABC免疫酶染色法对65例宫颈鳞癌、23例女阴疣状增生病(尖锐湿疣14例、乳头状瘤9例)的组织标本,进行了人乳头瘤病毒(HPV)属特异性抗原的检测。在宫颈鳞癌及女阴疣状增生病中HPV抗原的阳性率分别为13.84％(9/65)和52.17％(12/23)。HPV抗原阳性反应的表现有三种类型:(1)全核型:细胞核呈均匀深棕色反应;(2)核内包涵体样型:核内出现棕色不规则形的包涵体样结构;(3)核周颗粒型:在挖空细胞的核周空隙区内出现较细小的棕色颗粒。

ABC staining of enzyme immunoassay were used to assay specific antigen of human papilloma virus(HPV)in tissue samples collected from 65 cases of cervical ,squamou: carcinoma, and 23 cases of proliferative verrucous vaginal diseases (14 casea of condyloma acuminata, 9 cases of papilloma).The findings showed positive antigen reactions to HPV is 13.84%(9/65) of cervical squamous carcinama, and 52.17％(12/23)in the group of patients with proliferative verrucous vaginal disseases lespectively. The positive antigen reactions to HPV could be shown in three types: 1.pantonucleus type: whole nucleus showed homogeneous dark brown, 2.intranucleus cytorrhycte type:heterogeneous brown cytorrhyctes seen in the nucleus, 3.paranuclear granule type:small brown granules occurred in the paranuclear space of koilocyte.