雌激素受体ERβ配体结合区的异源表达与纯化

Heterologous expression and purification of the ligand binding domain of estrogen receptor β

为研究环境化合物与雌激素受体的相互作用,在大肠杆菌中异源表达了人雌激素受体ERβ的配体结合区(hERβ-LBD)蛋白。在诱导剂(IPTG)浓度为0.2mmol.L-1,培养温度25℃,诱导时间为8h的优化表达条件下,经亲和色谱法纯化后,hERβ(LBD蛋白的产量可达236mg.L-1.

In order to investigate the interaction between environmental chemicals and estrogen receptors,the ligand binding domain of human estrogen receptor β(hERβ-LBD) was expressed in Escherichia coli.Based on the optimized expression conditions(IPTG,0.2 mmol·L-1;culture temperature,25 ℃;time of induction,8 h),after purified by affinity chromatography,the production of hERβ-LBD can be reached to 236 mg·L-1.