水源水中2-MIB降解菌的筛选

Isolation of a bacterium capable of removing 2-methylisoborneol from water

针对特定水处理工艺,研究了投加2-MIB前后水中微生物种群的变化,建立了筛选分离2-MIB降解菌的方法.以生物膜生长成熟的活性炭连续处理含有较高浓度2-MIB水样,然后从活性炭表面取样,用2-MIB为惟一碳源的无机盐培养基筛选和驯养,最后进行划线分离,成功得到2-MIB降解菌种.通过16SrRNA分析,该菌株属于假单胞菌属(Pseudomonas spp.).

For the given water treatment,the features of the microbiological colonies before and after the addition of the 2-methylisoborneol(2-MIB) were studied,and a method for isolation of a bacterium capable of removing the 2-MIB from the water was proposed.The procedure of the method was as follows:the activated carbon,whose surface was covered by a mature microbiological membrane,was employed to continually treat the water with higher concentration of 2-MIB,and then the sample gained from the surface of the activated carbon was cultivated in the mineral salt liquid culture in which 2-MIB was the only carbon source;finally,one strain,which was identified as Pseudomonas spp.based on the data of 16S rRNA analysis,was isolated by plate streaks.