不同激素对卵母细胞体外成熟培养的影响

Effects of Different Originated Gonadotropin Hormones on In Vitro Maturation of Goat Oocytes

本试验对比在成熟液中添加不同来源的促性腺激素,即进口的和国产的促性腺激素(FSH+LH)对山羊卵母细胞体外培养成熟效果的影响。二者对山羊卵母细胞成熟均有促进作用,卵母细胞成熟率都极显著高于对照组(P<0.01),分别为67.3%、68.9%和15.6%,但是它们之间的成熟率差异不显著(P>0.05)。国产激素成本低,使用方便,在卵母细胞体外成熟培养时,完全可以用国产的促性腺激素代替进口的FSH和LH。

Comparison of the effects of FSH ＋LH from different origins （imported and domestic） on maturation of goat oocytes in vitro maturation was conducted in this study. The two compounds from different origins all promoted oocytes maturation, the maturation rates in the two groups （67.3 % and 68.9 % ） were all significantly higher than that of the control group （15.6%）, and there was no significant difference in the maturation rate for the two gonadotropin groups. By comparison, there were the same impact of the two gonadotropin compounds （FSH＋LH） from different origins on maturation of goat oocytes in vitro maturation （IVM）, but the domestic one cost much lower than imported one. So we may use the domestie FSH＋LH in goat oocyte IVM instead of the imported one.