细辛提取物对镰刀菌抑菌作用的初步分析

Inhibitory Effect of Asarum heterotropoides Against Fusarium spp.

为了研究细辛对镰刀菌的抑制作用及机理,采用生长速率法和孢子萌发法测定了细辛对镰刀菌的抑菌作用,采用电导法研究了细辛对镰刀菌细胞膜的通透性影响。结果表明:细辛对镰刀菌菌丝生长和孢子萌发有很好的抑制作用,且菌丝比孢子对细辛提取物的敏感性更强。电导率测定结果表明,细辛提取物处理的镰刀菌的电导率值显著高于对照组,说明了细辛提取物可以破坏镰刀菌的细胞膜选择通透性,导致内容物外渗,细胞膜是细辛提取物的作用靶点之一。

The inhibitory effect of Asarurn heterotropoides against Fusariurn, the toxicity on the mycelium and spores and cell membrane permeability of Fusarium hypha were studied in the paper. The results showed that the inhibitory effect of Asarurn heterotropoides on the Fusarium spp. is good. And the inhibitory effect of Asarurn heterotropoides against the growth of mycelium is more obvious to the growth of the spore. At the same time, the conductivity of cell membrane is obviously higher than control in the different time; the electrical conductivity rates is increased obviously with the medicine concentration enhancement and treat time lengthening. That sugqested that the cell membrane permeability were destroyed after treated with Asarurn heterotropoides and substances in the cell were leaked outside. All these results showed that cell membrane was the one of targets attacked by Asarum heterotropoides.