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小麦TaMvb2-Ⅱ基因的克隆 被引量：1

Cloneing of Wheat TaMyb2-Ⅱ Gene
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摘要 为了研究小麦转录因子基因TaMyb2-Ⅱ的功能,利用SalⅠ和SacⅠ双酶切的方法,构建了基因克隆及表达载体PEBV,获得了正义PEBV-35Sp-TaMyb2-Ⅱ-NOSter阳性克隆,实现了TaMyb2-Ⅱ基因的定向克隆。 In order to analyse the function of wheat TaMyb2- Ⅱ gene, clone and expression vector PEBV was constructed and sense PEBV-35Sp-TaMyb2-NOSter positive clone was obtained by Sal Ⅰ and Sac Ⅰ double digests. Thus the TaMyb2- Ⅱ was directional cloned.
作者 王爱萍 梁素明 宋长水 董琦 杨武德
机构地区 山西农业大学农学院 山西农科院遗传研究所 山西省农业技术推广站
出处 《山西农业大学学报（自然科学版）》 CAS 2009年第2期104-105,共2页 Journal of Shanxi Agricultural University(Natural Science Edition)
基金 山西省青年科技研究基金(2008021044) 山西农大科技创新基金(2006024) 山西农大博士科研启动金(412549)
关键词 小麦 TaMyb2-Ⅱ 克隆 Wheat TaMyb2-Ⅱ Clone
分类号 S512 [农业科学—作物学]
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参考文献5

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  • 4王爱萍,毛新国,景蕊莲,昌小平,杨武德.TaMyb2-Ⅱ基因在普通小麦(Triticum aestivum L.)及其近缘种中的单核苷酸多态性分析[J].作物学报,2006,32(12):1809-1816. 被引量：7
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二级参考文献20

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山西农业大学学报（自然科学版）
2009年 第2期

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