儿童急性淋巴细胞白血病nm23H1基因表达与sIL-2R水平的变化

Expression of nm-23H1 genes and the levels of sIL-2R in ALL of children

目的探讨nm23H1基因表达和sIL-2R水平变化在儿童急性淋巴细胞白血病(ALL)中的临床意义。方法用RT-PCR和ELASA法分别检测了42例儿童ALL骨髓细胞nm23H1基因表达情况和血清slL-2R水平。结果儿童ALL初治组nm23H1基因表达阳性率为66%,表达程度明显高于对照组(P<0.01),完全缓解(CR)组表达程度下降,阳性率降低,为10.7%,与正常对照组比较差异无统计学意义(P>0.05)。复发难治组表达明显增高,为71.4%,与初治组比较差异无统计学意义(P>0.05)。sIL-2R水平在初诊组升高,与正常对照组比较差异有统计学意义(P<0.05),CR组sIL-2R水平下降,复发时再次升高。观察nm23H1基因表达阳性组和阴性组sIL-2R水平,发现阳性组sIL-2R呈高水平,追踪治疗反应该组CR率低,易复发。结论nm23H1基因表达和sIL-2R水平在儿童ALL疾病发展过程中有明显变化,有助于判断儿童ALL疾病期和预示复发,高表达的nm23H1和高水平的sIL-2R是否与难治复发有关,有待进一步扩大样本研究。

Objective To explore the clinnic siganification of nm23H1 gene expression and the level of sIL-2R in All for children.Methods Expression of nm23H1 gene in bone marrow cells and the level of sIL-2R was determined in 42 patients ALL for children,respectively with RT-PCR and ELASA.Results The position rate of nm23H1 gene expression for children'ALL was higher than that of control group(66%), significantly higher in than that of control group(P<0.01),complete remission(CR)group was lower(10.7%) and replase group was high expression again(71.4%),It showed no significance compartion with primary di- agnosis group(P>0.05).The level of sIL-2R in positive group was higher than that of negative group,it had lower CR rate and replase easily.Conclusion nm23H1 gene expression and SIL-2R levels has siganificantly change in the disease course of children's ALL,it contributes to judge the disease course and replase in children's ALL,wether it relate to refactory and replase or not for the high expression of nm23H1 and high level of sIL-2R,it will be progressively studied to enlarge samples.