多发性骨髓瘤患者MMP-2及VEGF表达的研究

Studies of MMP-2 and VEGF expression in multiple myeloma patients

目的探讨基质金属蛋白酶-2(MMP-2)、血管内皮细胞生长因子(VEGF)水平与多发性骨髓瘤(MM)的病情进展的关系和临床意义。方法用酶联免疫吸附试验(ELISA)分别检测了24例MM患者和10例正常对照骨髓基质细胞(BMSC)培养上清液的MMP-2、VEGF水平。结果14例进展期MM组的MMP-2、VEGF表达水平显著高于10例稳定期MM组和10例正常对照组(P【0.05),稳定期MM组和正常对照组的MMP-2水平差异无统计学意义(P】0.05),稳定期MM组VEGF水平高于正常对照组(P【0.05)。9例进展期MM组的BMSC与人骨髓瘤细胞系细胞U266(终浓度2×10~5/ml)进行混合培养48 h后,其上清中MMP-2、VEGF水平明显增高,显著高于进展期MM、稳定期MM、正常对照组BMSC及稳定期MM BMSC+U266组的水平(均为P【0.05)。稳定期MM BMSC+U266组的VEGF水平显著高于进展期MM、稳定期MM、正常对照组BMSC(P【0.05),差异有统计学意义。MMP-2与VEGF两者呈正相关(r=0.925,P【0.05)。结论MMP-2、VEGF水平升高与MM的病情进展或发病有关,该过程可能需骨髓瘤细胞和骨髓基质细胞协同作用而完成。

Objective To explore the role and implication of matrix metalloproteinase-2(MMP-2) and vascular endothelial growth factor (VEGF)in multiple myeloma (MM).Methods Expression MMP-2 and VEGF secreted by bone marrow stromal cells (BMSC) of 24 MM patients were detected by ELISA.Results The level of MMP-2 and VEGF in 14 active MM patients were significantly higher than 10 stable MM pa- tients and 10 healthy subjects(P<0.05),The level of VEGF in stable MM patients were different from healthy subjects(P<0.05) When human MM cell lines U266 were adhered to BMSC of 9 active MM patients.The level of MMP-2 and VEGF in active and stable MM patients were significantly higher than BMSC alone (P<0.05). The expression level of MMP-2 was related to VEGF (r=0.925,P<0.05).Conclusion The increase of MMP-2 and VEGF activity produced by BMSC or induced by myeloma cells in these cocultures is associated with progress or pathogenesis of MM,and may be invoved with tumor invasion.