去甲斑蝥素对人类胃癌SGC-7901细胞凋亡相关基因表达的影响

Influence of norcantharidin on apoptosis-related gene expression of gastric cancer cell line SGC-7901

目的探讨去甲斑蝥素(NCTD)对人类胃癌 SGC-7901细胞凋亡相关基因表达的影响。方法实验分对照组、5-Fu 组、NCTD 组和5-Fu+NCTD 组,分别应用 MTT、流式细胞术、免疫组化SABC 法检测 NCTD 对体外培养胃癌 SGC-7901细胞的杀伤抑制率、凋亡率和 survivin、bcl-2、caspase-3的表达。结果 NCTD 对人类胃癌 SGC-7901细胞生长有抑制作用,呈剂量-时间效应关系;凋亡率由(8.30±1.49)%上升到(20.56±1.32)%;survivin 基因表达由(86.57±4.39)%下降到(26.11±2.27)%;bcl-2基因表达由(85.35±3.25)%下降到(30.26±1.83)%;caspase-3基因表达由(54.49±3.07)%上升到(92.78±2.47)%;与5-Fu 联合应用上述作用更明显。结论 NCTD 能抑制人类胃癌 SGC-7901细胞的生长;与5-Fu 具有协同作用,其机制可能与其诱导细胞凋亡,影响凋亡相关基因表达有关。

Objective To investigate the influence of Norcantharidin(NCTD)on apoptosis-related gene expression of gastric cancer cell line SGC-7901.Methods The experiment was divided into control group,5-Fu group,NCTD group and 5-Fu+NCTD group.The inhibitory rate,apoptosis rate and expression of survivin,bcl-2,and caspase-3 were detected by MTT assay,flow cytometry and SABC immunohistochemical method,respectively.Results NCTD showed that the inhibitory effect on growth of SGC-7901 cells with a dose-and time-effective dependent manner.The apoptotic rate increased from(8.30±1.49)% to(20.56± 1.32)%.The expressions of survivin decreased from(86.57±4.39)% to(26.11±2.27)% and bcl-2 from(85.35± 3.25)% to(30.26±1.83)%,while caspase-3 increased from(54.49±3.07)% to(92.78±2.47)%,5-Fu had the synergistic effects with NCTD.Conclusion NCTD can inhibit the growth of SGC-7901 cell lines.5-Fu had the synergistic effects with NCTD.The mechanism might correlate with induction of cell apoptosis via effect of the expression of apoptotic-related genes such as survivin,bcl-2 and caspase-3.