骨肉瘤组织环氧合酶-2、CD_(105)表达与预后

Expression of cyclooxypenase-2 and the relation between cyclooxypenase-2 and CD_(105)in osteosarcoma

目的研究骨肉瘤组织中环氧合酶-2(COX-2)与 CD_(105)表达及其与预后的关系。方法应用免疫组织化学染色的方法检测人骨肉瘤中 COX-2和 CD_(105)的表达,并计算 CD_(105)标记的微血管密度(MVD),同时对预后进行 COX 多因素生存分析。结果 COX-2在骨肉瘤中表达阳性率为77.5%,COX-2的表达与外科分期和肿瘤的转移密切相关(P<0.05);外科分期的Ⅰ期与Ⅱb期、Ⅲ期间的 MVD值的差异有统计学意义(P<0.05),而Ⅰ期与Ⅱa期、Ⅱa期与Ⅱb期之间的差异无统计学意义(P>0.05);转移组的骨肉瘤其 MVD 值高于未转移组,二者之间的差异有统计学意义(P<0.05);COX-2表达与 MVD 值呈正相关(P<0.05)。多因素生存分析显示,Enneking 外科分期、COX-2表达及是否发生转移是影响患者预后的独立危险因素。结论 COX-2在骨肉瘤组织中呈现高表达,其高表达可能促进了骨肉瘤新生血管的生成。COX-2和 CD_(105)标记的 MVD 可以作为骨肉瘤外科分期和是否发生转移的重要指标。

Objective To study the expression of cyclooxypenase-2(COX-2),prognosis of patients and the correlation between COX-2 and CD_(105)in osteosarcoma.Methods The expression of COX-2 and CD_(105)in human osteosarcoma was detected with immunohistochemistry.Then to count the microvessl density (MVD)were marked with CD_(105),The prognosis of the patients with osteosarcoma was analyzed by Cox multi- variate survival analysis.Results The positive rate of COX-2 expression was 77.5 %,The expression of COX-2 was positively correlated with surgical grade and metastasis of osteosarcoma;There were significant difference between surgical grade Ⅰ and Ⅱ b、grade Ⅰ and Ⅲ(P<0.05),No significant difference was found be- tween grade Ⅰ and Ⅱ a,grade Ⅱ a and Ⅱ b(P>0.05);Metastatic cases had higher MVD than those without metastasis(P<0.05).The positive correlation was found between expression level of COX-2 and MVD marked by CD_(105).According to multivariate survival analysis,surgical stage of Enneking,COX-2 expression and metastasis of osteosarcoma were independent prognostic factors for poor prognosis.Conclusion The overex- pression of COX-2 is found in human osteosarcoma;Angiogenesis in osteosarcoma may be promoted by COX- 2.The COX-2 and MVD marked by CD_(105)can be considered as an important index which is associated with surgical grade and metastasis of osteosarcoma.