Rb、p53基因与PCNA在膀胱癌中异常表达的意义

Significance of abnormal expression of Rb,p53 and PCNA in patients with bladder carcinoma

目的检测膀胱癌中 Rb、p53基因及核细胞增生抗原(PCNA)的表达,探讨其与膀胱癌病理分级、临床分期及预后的关系。方法采用免疫组织化学方法检测60例老年人膀胱癌组织中 Rb 基因、突变型 p53基因及 PCNA 的表达。结果 60患者中,p53、PCNA 阳性表达者均为24例(40%),Rb 异常表达者27例(45.0%);30例(50.0%)膀胱癌患者同时有多个基因的表达异常。多种基因的异常表达与膀胱癌的病理分级、临床分期及复发有密切关系。结论肿瘤的多基因分析比单基因分析更有价值。Rb 基因、p53基因及 PCNA 异常表达及协同作用可能在膀胱癌的发生、发展中起重要作用。

Objective To discuss the significance of abnormal expression of Rb,p53 and PCNA in patients with bladder carcinoma and the correlation with the tumor grade,clinical stage and relapse.Meth- ods Immunohistochemical method was used to detect the Rb,p53 and PCNA gene expression in 60 cases with bladder carcinoma.Results The positive rates of p53,PCNA in 60 cases of bladder cancer is both 40.0 %.There were 27(45 %)cases lack of Rb expression.Multiple altered expression was found in 30 cases (50.0 %).The abnormal expression of Rb,p53 and PCNA was closely related to the tumor grade,clinical stage and relapse.Conclusion The detection of multiple gene is more valuable than that of single gene. Multiple altered expression of Rb,p53 and PCNA and their synergistic action might play an important role in the genesis and development of bladder cancer.