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Study on an electrochemical biosensor for thrombin recognition based on aptamers and nano particles

Study on an electrochemical biosensor for thrombin recognition based on aptamers and nano particles
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摘要 This paper presents a high specific, sensitive electrochemical biosensor for recognition of protein such as thrombin based on aptamers and nano particles. Two different aptamers were chosen to construct a sandwich manner for detecting thrombin. Aptamer I was immobilized on nano magnetic particle for capturing thrombin, and aptamer II labled with nano gold was used for detection. The electrical current generated from gold after the formation of the complex of magnetic particle, thrombin and nano gold, and then an electrochemical cell designed by ourselves was used for separating, gathering, and electrochemical detecting. Through magnetic separation, high specific and sensitive detection of the target protein, thrombin, was achieved. Linear response was observed over the range 5.6×10-12―1.12×10-9 mol/L, with a detection limit of 1.42×10-12 mol/L. The presence of other protein as BSA did not affect the detection, which indicates that high selective recognition of thrombin can be achieved in complex biological samples such as human plasma. This paper presents a high specific, sensitive electrochemical biosensor for recognition of protein such as thrombin based on aptamers and nano particles. Two different aptamers were chosen to construct a sandwich manner for detecting thrombin. Aptamer I was immobilized on nano magnetic particle for capturing thrombin, and aptamer II labled with nano gold was used for detection. The electrical current generated from gold after the formation of the complex of magnetic particle, thrombin and nano gold, and then an electrochemical cell designed by ourselves was used for separating, gathering, and electrochemical detecting. Through magnetic separation, high specific and sensitive detection of the target protein, thrombin, was achieved. Linear response was observed over the range 5.6×10?12?1.12×10?9 mol/L, with a detection limit of 1.42×10?12 mol/L. The presence of other protein as BSA did not affect the detection, which indicates that high selective recognition of thrombin can be achieved in complex biological samples such as human plasma.
出处 《Science China Chemistry》 SCIE EI CAS 2007年第3期351-357,共7页 中国科学(化学英文版)
关键词 aptamers NANO PARTICLES PROTEIN ELECTROCHEMICAL sensor aptamers nano particles protein electrochemical sensor
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参考文献28

  • 1Stojanovic M N,Landry D W.Aptamer-based colorimetric probe for cocaine. Journal of the American Chemical Society . 2002
  • 2Brody E N,Gold L.Aptamers as therapeutic and diagnostic agents. Reviews in Molecular Biotechnology . 2000
  • 3Pavlov V,Xiao Y,Shlyahovsky B,Willner I.Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin. Journal of the American Chemical Society . 2004
  • 4Murphy M B,Fuller S T,Richardson P M,Doyle S A.An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Research . 2003
  • 5Maxwell D J,Taylor J R,Nie S.Self-assembled nanoparticle probes for recognition and detection of biomolecules. Journal of the American Chemical Society . 2002
  • 6Ho H A,Leclerc M.Optical sensor based on hybrid ap- tamer/conjugated polymer complexes. Journal of the American Chemical Society . 2004
  • 7Bullock T L,Sherlin L D,Perona J J.Tertiary core rearrangements in a tight binding transfer RNA aptamer. Nature Structural Biology . 2000
  • 8Tombelli S,Minunni M,Mascini M.Analytical applications of ap-tamers. Biosensors and Bioelectronics . 2005
  • 9Fields S.Proteomics in genomeland. Science . 2001
  • 10Kawde A N,Rodriguez M C,Lee T M H,Wang J.Label-free bio- electronic detection of aptamer―protein interactions. Electrochemistry Communications . 2005

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