特异性血小板抗体检测的实验研究

Study on the method for detection of specific platelet antibody

目的 建立一种简易、特异性强的血小板抗体检测方法。方法 利用磷酸氯喹溶液对血小板进行处理 ,去掉血小板相关抗原 (HLA抗原 ) ,用只含特异性抗原的血小板包被聚乙烯板来捕获血小板抗体。结果 与常用的SEPSA和淋巴细胞毒实验 (LCT)比较 ,该方法只表现血小板特异性抗体反应。结论 本方法能够鉴别血小板相关抗原的抗体和血小板特异性抗原的抗体 ,为临床血小板减少疾病的鉴别诊断。

Objective To establish a high specific and simple method for detection of platelet antibody.Method To treat platelet using Chloroquine Diphosphate salt and do awaywith its platelet associated antigens(HLA-antigen),then capture platelet antibodies using U-plate coated with platelet containing specific antigen.Results Compared with usually methods such as SPESA and LCT,the above method only indicate specific antibody reaction in platelet.Conclution The above method can identify antibodies between platelet-associated antigens and platelet-specific antigens,which can be used in the clinical diagnosis on platelet-decreasing disease and suitable as an useful supplement technique for explaining no-haemolytic fever and ineffective platelet transfusion.