野生绞股蓝复方预防高胆固醇饵料兔肝纤维化研究

Research of Wild Gynostemma Pentaphyllum Prescription to Prevent Liver Fibrosis of Rabbits fed with Cholesterolrich Diet

目的 探讨野生绞股蓝复方对高胆固醇饵料兔肝纤维化的预防作用。方法 实验兔分为A组 (草食 )、B组 (高胆固醇饵料 +野生绞股蓝复方 )、C组 (高胆固醇饵料 +辛伐他丁 )、D组 (高胆固醇饵料 ) 4组。比较饲养前后组内及饲养后组间透明质酸 (HA)、层黏蛋白 (LN)、前胶原Ⅲ (PCⅢ )、血脂、肝功能的变化。结果 饲养 4个月后B组HA为 (13 0 1± 8 2 )ng/ml,LN为 (15 4 2± 14 1)ng/ml,PCⅢ为 (2 4 4± 7 1)ng/ml ,与饲养前比较差异有显著性 (P <0 0 5 ) ;C组HA为 (2 40 2± 14 1)ng/ml ,LN为 (2 69 7± 2 6 8)ng/ml,PCⅢ为 (4 0 1± 8 4)ng/ml ,与饲养前比较差异有非常显著性 (P <0 0 1) ;C组各指标均高于B组 ,两组比较差异有显著性 (P <0 0 5 )。饲养后B、C组胆固醇上升 ,与饲养前比较 ,差异有显著性 (P <0 0 5 ) ,B、C组组间胆固醇比较差异无显著性 (P >0 0 5 ) ;D组胆固醇上升 ,与饲养前比较 ,差异有非常显著性 (P <0 0 0 1)。B组甘油三脂上升 ,与饲养前比较差异有显著性 (P <0 0 5 ) ,C组甘油三脂上升 ,与饲养前比较差异有非常显著性 (P <0 0 1) ,C组甘油三脂高于B组 ,差异有显著性 (P <0 0 5 ) ,D组甘油三脂上升 ,与饲养前比较差异有非常显著性 (P <0 0 0 1)。

Objective Effects of Wild Gynostemma Pentaphyllum Prescription to prevent liver fibrosis of rabbits fed with cholesterolrich diet Methods The rabbits for experiment are divided into groups called A、B、C and D The rabbits in group A was fed with grass B、C and D was fed with cholesterolrich diet we want to set up some animal model with high bloodlipida dn fatty liver Group B was given Wild Gynostemma Pentaphyllum Prescription,group C was given simvaltadine The differences and changes of HA、LN、PCⅢ、bloodlipid、ALT and TBiL were tested and compared before and after four groups were fed The liver was checked with pathology Results After being fed,HA、LN、PCⅢ in group B were(130 1±8 2)ng/ml,(154 2±14 1)ng/ml,(24 4±7 1)ng/ml,there was difference( P <0 05),in group C were(240 2±14 1)ng/ml,(269 7±26 8)ng/ml,(40 1±8 4)ng/ml,difference was significant( P <0 01) After being fed,HA、LN、PCⅢ in group B are lower than those in group C,there was difference( P <0 05) After being fed,ALT、TBiL in group B had no obviously chang,in group C and Draised,there was difference( P <0 005),difference was significant( P <0 01) Conclusion Wild Gynostemma Pentaphyllum Prescription had function of preventing liver fibrosis of rabbits fed with cholesterolrich diet