分泌型与包涵体型G-CSF对骨髓干细胞动员效率的比较

目的 观察 2种不同G CSF动员剂 (分泌型与包涵体型G CSF) ,对急性心肌梗死 (AMI)患者外周血干细胞动员的效率。方法 A组予以包涵体型G CSF(商品名 :惠尔血 ) ,30 0 μg ,每日 2次 ,皮下注射 ,连续 5d ;B组予以分泌型G CSF(商品名 :金磊赛强 ) ,30 0 μg ,每日 2次 ,皮下注射 ,连续 5d。第 6天经美国Baxter公司生产的CS30 0 0PLUS血细胞分离机 ,分离外周血干细胞 ,采集外周血干细胞悬液经流式细胞仪测定CD34+ 的细胞数量。结果 两组在用药前及用药后第 3、4、5、6天外周血中CD34+ 细胞数量和WBC计数无明显统计学差异 ;在应用两组不同的动员剂后 ,外周血中WBC计数与动员时间变化曲线显示曲线高峰在动员后第 5天 ;在A组CD34+ 细胞数量与时间变化曲线高峰为第五天 ,但在B组CD34+细胞数量与时间变化曲线显示CD34+ 细胞数量在 3～ 4d内呈急剧升高趋势 ,但在第 5天后升幅明显减缓 ;显示B组动员后外周血中干细胞下降较慢 ;患者外周血中CD34+ 细胞数量与WBC计数变化呈正相关 (r=0 .835 ) ,与性别、体重、年龄及AMI发生时间无相关性。结论 在AMI患者中应用 2种不同G CSF动员剂 (分泌型与包涵体型G CSF) 。

Objective Observe the mobilization effects of autologous circulating blood stem cell with different type of G-CSF in patients with acute myocardial infarction (AMI).Methods 45 patients with AMI were randomly allocated to receive either inclusive type Granulocyte Colony-Stimulating Factor (G-CSF), or excrete type G-CSF to mobilize the stem cell. The patients receive the dose of G-CSF 600 μg/day , by hypodermic injection ,and the duration of applying G-CSF was 5 days . In the process of the mobilization of the circulating blood stem cell, the white blood cell (WBC) and CD34 + cell count in the circulating blood should be observed. Results Prior to applying G-CSF and the 3rd、4th、5th、6th after applying G-CSF, the counts of WBC and CD34 + cell have no significant difference between this two groups ; the peak of WBC count was at the 5th after applying G-CSF; while the count of CD34 +cell has increased rapidly after 3rd ,and still increase lowly at the 5th after applying G-CSF; the counts of CD34 + cell had no significant difference between this two groups at 7th(P=0.333); the count of CD34 + cell in the circulating blood was positive referent with the count of WBC in the circulating blood(r=0.835),was not reference with sex、age、 body weight、and the onset time of AMI. Conclusions In patients with AMI, the mobilization effects of the circulating blood stem cell with applying with inclusive type G-CSF or excrete type G-CSF was not significant different.