降解纤维素的真菌分离、筛选及其酶活测定

Separate and Screen Fungus Which can Biodegrade Cellulose andAssay Its Enzyme Activity

在自然界环境和活性污泥中筛选出4种分解纤维素能力较强的真菌,并将这4种菌株单独或以不同的组合形式在羧甲基纤维素钠(CMC＿Na)为惟一碳源的产酶培养基中进行产酶特性的研究。混合菌种在发酵培养基中培养144h后达到产酶高峰,酶活力最高可达到33μmol mL,超过菌种的单独培养。这4种菌均对李坑污水有一定的降解能力,若能继续进行研究,对实际应用有一定前景。

This experiment separated four types of fungus which can biodegrade cellulose from natural environment and active sludge, then putted them and their different combinations into the culture medium which only use CMC_Na as silicon source, studied their zymogenic characteristics.After 144 hours the fungous combinations achieved their peak zymogenic production,enzyme activity come to 33 μmol/mL,much more than the single fungi. These four types of fungus can also biodegrade polluted water of Likeng, if we continue to research them,they could be used to treat polluted water.