枣庄市致病性弧菌及肠毒素检测研究

Study on Pathogenic Vibrio and Detecting Toxin of Pathogenic Vobrio in Zaozhuang City

目的 探讨枣庄市致病性弧菌的种类,分布,构成特征,并对致病性弧菌进行肠毒素检测。方法 1990～1998年我们采用生化反应VBO血清,对非01群霍乱弧菌进行血清分型,通过生化反应对所检其他致病性弧菌进行鉴定,应用反向血凝法和ELISA法检测肠毒素。结果 9年共检出7种致病性弧菌共291株。以非01群霍乱弧菌为主,占49.83％。类霍乱肠毒素(CT)和耐热肠毒素(NAG-ST)检出率分别为51.20％和44.33％。结论 摸清我市致病性弧菌分布情况,我市致病性弧菌以非01群霍乱弧菌为主。提出类CT是引起急性腹泻一个重要致病因子。应用ELISA法检测NAG-ST方法简单,效果较好。

Objective To find out the type., distributions composition of pathogenic Vibrio, and detect the toxin of pathogenic Vibrio. Methods From 1990 to 1998 we typed Vibrio cholerase serogroup non by using VBO blood type. Detected pathogenicty of pathogenic Vibrio by biochemical reaction. Detected cholera enterotoxin by using RPHA and ELISA Results The amount of pathogenic Vibrio is 291 and belong to 7 type. The main part of it is Vibrio cholerase serogroup non in Zaozhuang and it is rate is 49.83 percent.The detect rate of CT and ST is 51.20 percent and 44.33 percent. Conclusion The main pathogenic Vibrio is Vibrio cholerase serogroup non in Zaozhuang and CT is a important factor in diarrhoea, and it is simple to detect NAG - ST by using ELBA.