Effect of aloe-emodin on expression of proliferating cell nuclear antigen of vascular smooth muscle cells in culture after arterial injury

芦荟大黄素对动脉损伤后平滑肌细胞PCNA基因表达的影响(英文)

Objective To observe the effect of aloe-emodin on the expression of proliferating cell nuclear antigen (PCNA) in smooth muscle cells (SMCs) after arterial injury and study the molecular mechanism of inhibition of aloe-emodin on SMC proliferation.Methods Deendothelialization was performed at the abdominal aorta in Japanese white rabbits using a 3F Fogarty arterial embolectomy catheter. 48 hours later, the medium of abdominal aorta was isolated and primary SMCs culture was performed. Cells were synchronized to G0 by serum starvation, then aloe-emodin at a concentration of 20?μg/ml was added to the culture medium containing 10%［v/v］ fetal calf serum. Vehicle was also added to the medium as a control. After 18 hours, the expression of PCNA at the level of mRNA and protein were examined using techniques of RT/PCR, Western blotting and inmmunocytochemistry respectively. Results Compared with the control group, the expression of PCNA mRNA and protein was prominently decreased after addition of aloe-emodin. Conclusion The inhibition of aloe-emodin on SMCs proliferation may be caused by inhibiting the expression of the PCNA gene.

目的 通过观察芦荟大黄素对动脉损伤后平滑肌细胞增殖细胞核抗原 (PCNA)基因及蛋白表达产物的作用 ,探讨芦荟大黄素抑制平滑肌细胞增殖的分子作用机制。方法 用 3FFogarty球囊拉栓导管对纯种日本大耳白兔腹主动脉进行球囊内皮剥脱损伤 ,4 8小时后取出腹主动脉中膜进行原代平滑肌细胞培养 ,细胞同步于G0 /G1期后 ,实验组加芦荟大黄素 2 0 μg/ml,对照组加等体积的细胞培养液 ,18小时后 ,分别用RT/PCR、Western杂交法检测药物组与对照组平滑肌细胞PCNA基因mRNA和蛋白表达产物的变化。结果 与对照组比较 ,加用芦荟大黄素以后 ,平滑肌细胞PCNAmRNA和蛋白水平上的表达均显著抑制。结论 芦荟大黄素对平滑肌细胞的增殖抑制作用可能是通过影响PCNA基因的表达实现的。