慢性肾功能衰竭患者血清粒系集落刺激因子水平观察(英文)

Serum granulocyte colony-stimulating factor in patients with chronic renal failure

目的 探讨粒系集落刺激因子 (G CSF)的调节机制及代谢途径。方法 本文运用酶联免疫吸附试验 (ELISA)检测 61例慢性肾功能衰竭 (CRF) +/ 血液透析患者血清G CSF水平。结果 CRF患者血清G CSF水平明显升高。CRF非血透患者血清G CSF检出率为 80 65% ,血清G CSF水平为566 4 0± 2 0 7 98ng/L。CRF血透患者G CSF检出率为 93 33% ,血透前和血透后G CSF水平分别为 12 55 36±611 2 5ng/L和 1151 61± 599 4 7ng/L。血透组G CSF水平略高于非血透组 ,但两组无明显差异。血透前与血透后患者G CSF水平无明显差异。进一步分析表明 ,G CSF水平与WBC ,BUN或Scr水平无关 (P >0 0 5)。结论 CRF患者血清G CSF水平升高可能是由于肾功能不全导致G CSF清除减少或 /和G CSF产生增加。

Objective To gain a better understanding of the regulatory mechanism and kinetic behaviour of granulocyte colony-stimulating factor (G-CSF).Methods An enzyme-linked immunosorbent assay (ELISA) method was used to detect serum G-CSF in 61 patients with chronic renal failure±long-term hemodialysis and 30 normal controls. Results Serum G-CSF levels in CRF patients were significantly higher than in normal controls. Eighty percent of patients had detectable G-CSF and serum G-CSF levels were 566.40±207.98?ng/L in non-hemodialyzed (non-HD) patients. The detectable percentage in hemodialyzed patients was 93.33%, serum G-CSF levels in pre-HD and post-HD patients were 1255.36±611.25?ng/L and 1151.61±599.47?ng/L respectively. Serum G-CSF levels in HD patients were slightly higher than in non-HD patients, but no significant difference was found between the two groups. No difference was found between the G-CSF values obtained in pre-HD and post-HD patients. There was no relationship between G-CSF levels and WBC, BUN or Scr (P>0.05).Conclusion The high value of G-CSF in patients with CRF may be caused by a decrease in G-CSF clearance and/or an increase in G-CSF release.