摘要
Objective To study the relationship between apoE alleles and life-span. Methods Apolipoprotein E genotypes were determined in 164 unrelated Uygurs including 35 persons aged 90 years or older, 71 men aged 20-35 and 54 men with myocardial infarction by using polymerase chain reaction-restriction fragment length polymorphism. Apolipoprotein E gene polymorphism was analyzed among three groups. Results There was statistically significant difference in the ε4 allele frequencies among three groups. The ε4 allele frequency in olds was the lowest (0.057), while in patients suffering from myocardial infarction (MI) was the highest (0.213). In MI patients the average age of first attack in ε4 allele carriers was significantly younger than that of non-carries, 51.3 and 58.3 years, respectively (P<0.05). Conclusion ApoE genotype may have some impact on human longevity.
目的 探讨载脂蛋白E(apoE)基因多态性与人类寿命的关系。方法 采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)分析技术 ,对 35名维吾尔族长寿老人 ,71名维吾尔族青年男性和 5 4名维吾尔族男性心肌梗塞病人的apoE基因频率分布特点进行了对比分析。结果 维吾尔族长寿老人ε4等位基因的频率最低 0 0 5 7,普通青年男性居中 0 12 7,心肌梗塞病人最高 0 2 13,三组比较存在统计学差异 (LRχ2 =9 377,ν=2 ,P =0 0 0 9)。不仅如此冠心病人中ε4等位基因携带者心肌梗塞发病年龄为 5 1 3,而非携带者心肌梗塞发病年龄为 5 8 3,差异存在统计学意义 (P <0 0 5 )。结论 ε4等位基因携带者的平均寿命低于其他等位基因携带者。
