摘要
Objective To assess the relationship between apolipoprotein E (apoE) polymorphism and the early onset of coronary heart disease (CHD) and the effect of apoE on lipids and lipoproteins in healthy Chinese subjects. Methods Sixty-eight patients with CHD younger than 55 years (CHD1), 136 patients with CHD older than 65 years (CHD2), and 136 healthy subjects were enrolled, and their plasma levels of triglyceride (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were determined. The apoE genotypes were identified by polymerase chain reaction-restriction fragment length polymorphism. Results apoE 3/4 genotype and E4 allele frequency in the CHD1 group were higher than those in the CHD2 group and healthy subjects, while no differences were found between CHD2 and healthy subjects. Meanwhile, the plasma levels of TC and low density lipoprotein cholesterol (LDL-C) were higher in the CHD2 group than in both CHD1 group and healthy subjects.Each apoE isoprotein has variable TC and LDL-C levels that is E2 (E2/2+E2/3)＜E3(E3/3)<E4(E4/4+E3/4). Conclusion apoE is one of the genetic factors that affect TC and LDL-C levels, and apoE 4 has a very close relation to CHD, suggesting that apoE 4 is an independent genetic factor of the early onset of CHD.
目的 探讨apoE基因多态性与早发冠心病患者的关系以及对中国人血脂水平的影响。方法 选择 68例年龄≤ 5 5岁的冠心病患者 (CHD1)、136例年龄≥ 65岁的冠心病患者 (CHD2 )及 136例健康对照者 ,测定空腹血清甘油三酯 (TG)、总胆固醇 (TC)和高密度脂蛋白胆固醇 (HDL C)水平 ;应用PCR ,HhaI内切酶消化及PAGE的方法确定apoE基因多态性。结果 apoE3/4基因型频率和E4 等位基因频率在CHD1组明显高于CHD2 和健康对照组 ,CHD2 组和健康对照组无差别。CHD1与对照组血脂水平无差别 ,而CHD2 组的TC、LDL C明显高于CHD1组和健康组。不同的apoE基因型TC、LDL C的浓度存在显著性差异 ,按E2 (E2 /2 +E2 /3) <E3(E3/3) <E4 (E4 /4+E3/4)递增。结论 apoE多态性是影响TC、LDL C水平的重要遗传因素之一 ;apoE4 与CHD有明显的相关性 ,即apoE4 可能是早发冠心病独立的易患遗传危险因素。
