Immune privilege induced by cotransplantation of islet and allogeneic testicular cells

胰岛与FasL^+睾丸细胞共移植产生免疫豁免(英文)

Objective To induce islet allograft long-term survival through cotransplantation of islet cells with sertoli cells.Methods Testicular sertoli cells were prepared by digestion with collagenase, trypsin and DNase, and were cultured for 48 hours. Collagenase digested and Ficoll purified donor (Wistar rat) islets were cotransplanted with allogeneic sertoli cells in the absence of systemic immunosuppression. Terminal leoxynucleotidyl transferase-mediated X-dUTP nick-end labeling (TUNEL) was used to label apoptosis of lymphocytes surrounding the islet graft.Results Cotransplantation of islets and 1 × 107 sertoli cells reversed the diabetic state for more than 60days in 100% (6/6) of the chemically diabetic Sprague Dawley rats. Grafts consisting of islets alone or islets plus 1 × 105 sertoli cells survived only for 5 - 6 days. Apoptosis of lymphocytes surrounding the islets was quite clear.Conclusion Cotransplantation of islets with FasL+ sertoli cells induces local immune privilege and allows long-term graft survival without systemic immunosuppression.

目的 FasL (Fas配体 )的表达对免疫豁免部位及免疫豁免组织的形成起重要作用。本研究通过胰岛与睾丸细胞共移植诱导胰岛移植物的长期生存。方法 酶消化制备睾丸sertoli细胞培养 4 8小时 ;胶原酶消化、Ficoll梯度离心纯化的供体 (Wistar大鼠 )胰岛 5 0 0个与培养后的睾丸sertoli细胞共移植。移植前后不用全身性免疫抑制剂。TUNEL法标记胰岛移植物周围凋亡的淋巴细胞。结果 胰岛与 1× 10 7睾丸sertoli细胞共移植可以纠正链脲酶素所致的糖尿病高血糖状态达 60天 (有效率达10 0 % ,6/6)。单纯的胰岛移植或与 1× 10 5睾丸sertoli细胞共移植 ,移植物仅能存活 5 - 6天。移植物周围的淋巴细胞可见明显的凋亡存在。结论 胰岛与FasL+ 睾丸细胞共移植可以诱导局部免疫豁免 ,产生移植物长时间存活。