Modified cold storage of rat livers with self-made HYD solution

自制HYD液低温保存鼠肝方法的改进

Objective To investigate the cold preservation effect on rat livers of a modified storage method with self-made HYD solution.Methods The vascular bed of rat livers was expanded with an additional 20 to 40?ml self-made HYD solution/100?g liver. After resection of the liver, the extra HYD solution (expressed as % liver weight) was entrapped via portal infusion by tying off the supra- and infra-hepatic inferior vena cava. Forty rats were randomly divided into four groups including control group with conventional storage method, and 20%, 30% and 40% groups according to the amount of extra HYD solution. We compared the preservation effect of the modified storage method with that of the conventional storage method using an isolated perfused rat liver model.Results Bile production and all the indices of hepatic microcirculation including portal perfusion pressure, endothelin-1 in the effluent, trypan blue distribution time and histology were significantly superior in the modified method groups compared to those in the control group (P<0.05). The contents of dihydroxybenzoic acid (DHBA) in the modified method groups were significantly lower than those in the control group (P<0.05). Liver enzymes activities in the 30% group were markedly lower than those in the control group (P<0.05). The preservation effect on rat liver in the 30% group was the best among the modified method groups.Conclusion The modified cold storage method is effective and may have potential for clinical application in liver preservation.

目的 为探讨改进大鼠肝脏低温保存方法对鼠肝的影响 ,将切取后的鼠肝灌入一定量的自制保存液 (HYD液 )后 ,结扎进出肝脏的各血管。方法 应用大鼠离体肝灌注模型 ,对传统保存法 (对照组 )和改进保存法 (实验组 :2 0 %组、30 %组和 4 0 %组 )肝脏微循环指标 (门静脉灌注压、流出液内皮素 1、台盼蓝分布时间及组织学改变 )、流出液有关酶指标水平及分泌胆汁量进行了观察。结果 2 0 %组、30 %组及 4 0 %组肝脏微循环指标及分泌胆汁量明显优于对照组 (P <0 0 5) ,30 %组肝脏有关酶指标水平明显低于对照组 (P <0 0 5) ,其中 30 %组保存肝脏效果最佳。结论 改进法保存鼠肝的各项指标明显优于传统法 ,故提示本法行之有效 ,值得临床工作借鉴。