摘要
Objective To investigate what effects BmKAS 1 (a polypeptide purified from the Chinese scorpion Buthus martensi Karsch [BmK] and named as BmK activator of skeletal muscle ryanodine receptor) and its upstream mixture BmK1 3 2 have on Na + channels in dorsal root ganglion (DRG) small diameter neurons Methods The whole cell patch clamp technique was used to investigate the effects of BmKAS 1 and BmK1 3 2 on Na + current in rat small diameter DRG neurons Results About 50% peak Na + current was suppressed by 10?μg/ml of BmK1 3 2 1 62?μg/ml of BmKAS 1 also blocked 50% peak Na + current, and there was an obvious dose dependent relationship Conclusion Both BmK1 3 2 and BmKAS 1 have a blocking effect on Na + channels, and this may one of the mechanisms for the analgetic effect of BmK1 3 2 and BmKAS 1
目的 研究新的蝎毒素纯化多肽BmKAS 1及其前级提取物BmK1 3 2对大鼠背根神经节小直径神经元钠通道电流的作用 ,并探讨两者与其外周镇痛作用间的关系。方法 运用全细胞膜片箝技术观察了BmKAS 1和BmK1 3 2对钠电流的作用。结果 10 μg/ml的BmK1 3 2可阻断约 50 %的峰钠电流 ,而 1 6 2 μg/ml的BmKAS 1即可达到相同的阻断效果 ,同时BmKAS 1对钠电流的作用呈现出明显的剂量反应关系。结论 BmKAS 1和BmK1 3 2对钠电流均有较强的抑制作用。鉴于钠通道与损伤所致的外周神经元感觉过敏有关 ,这种抑制作用可能是BmKAS 1和BmK1 3
基金
ThisprojectwassupportedbytheNationalNaturalScienceFoundationofChina (No 3 9870 70 2 )
theNationalDrugFoundationofChina(No
