Growth inhibition of interleukin-2 receptor gene-transduced peripheral blood lymphocytes on human ovarian cancer cells

目的 为研究IL 2R基因转染PBL对人卵巢癌细胞生长抑制的影响。方法 用脂质体Fugene作载体将IL 2和IL 2R基因分别转染入人卵巢癌细胞株 3AO和人外周血白细胞 (PBLs) ,转染 2 4小时后分别将转染和非转染的PBLs与转染和非转染的 3AO细胞混合培养 4 8小时 ,并用MTT法测定PBLs对 3AO细胞的杀伤作用。结果 转染前后 3AO细胞和PBLs细胞形态无明显变化。 3AO能转染IL 2基因并表达IL 2mRNA ,PBLs能转染IL 2R基因并表达IL 2RmRNA。IL 2基因修饰 3AO细胞可增强淋巴细胞对 3AO细胞的抑制作用 ,同时联合IL 2R基因转染PBLs和IL 2基因修饰 3AO细胞可显著增强其淋巴细胞对 3AO细胞的抑制作用。结论 IL 2R基因转染效应细胞可增加IL 2基因转染肿瘤细胞的抗肿瘤免疫效应 ,这一方法为卵巢癌IL

Objective To investigate the growth inhibition of interleukin 2 receptor (IL 2R) gene transduced peripheral blood lymphocytes (PBLs) on human ovarian cancer cells Methods Interleukin 2 (IL 2) and IL 2R genes were transfected into human ovarian cancer cell line 3AO and PBLs, respectively, using the same Fugene vector Twenty four hours later transfected and nontransfected PBLs were cocultured with transfected and nontransfected 3AO for 48 hours Cytotoxity of PBLs on 3AO was detected by the MTT assay Results The morphology of IL 2 transduced 3AO and IL 2R transduced PBLs remained unchanged 3AO cells could be transfected with the IL 2 gene and expressed IL 2 mRNA, and PBLs could be transfected with the IL 2R gene and expressed IL 2R mRNA IL 2 transduced 3AO cells enhanced their response to the cytotoxity of PBLs Furthermore, growth inhibition of PBLs to 3AO cells increased significantly when the IL 2R was transfected into PBLs and when the IL 2 gene was transfected into 3AO cells and the two were combined Conclusions IL 2R gene transduced PBLs are able to enhance their cytotoxity on IL 2 gene transduced ovarian cancer cells This method may be a new way to investigate IL 2 gene therapy for ovarian cancer