摘要
Objective To clarify the distribution of hepatitis A virus (HAV) genotype in geographical regions of China Methods Seventeen representative HAV strains were isolated from the stool or serum of hepatitis A patients in different geographical regions Viral RNA was recovered from stool or serum by proteinase K digestion and phenol chloroform extraction, followed by ethanol precipitation prior to reverse transcription and polymerase chain reaction (RT PCR) amplification The nucleotide sequences of VP1/2A junction region were tested by using a direct sequencing technique Results A pairwise comparison of sequences within 168 bases at the VP1/2A junction revealed that all the sequences clustered within genotype Ⅰ About 53% of strains clustered in genotype ⅠB, with less than 6% variability; while the others clustered in genotype ⅠA, with less than 5 3% variability Sequence homology between genotype ⅠA and ⅠB varied from 88 7% to 92 3% Conclusion Epidemic or sporadic HAV strains in China may belong to HAV genotype ⅠA or ⅠB Epidemiologically related strains may be identical or closely related in sequence
目的 了解甲型肝炎病毒 (HAV)在中国几个城市的基因型分布 ,为HAV的分子流行病学追踪调查提供方法和依据。方法 17株HAV代表株分别来自不同城市的甲型肝炎病人粪便或血清 ,病毒RNA经蛋白酶K消化、酚 /氯仿提取和乙醇沉淀后 ,以逆转录 套式聚合酶链反应 (RT PCR)扩增合成HAVVP1/ 2A交接区基因区 ,并进行直接核苷酸序列分析和差异比较。结果 VP1/ 2A交接区核苷酸序列分析表明 ,所有病毒株均从属于基因Ⅰ型 ;约 53%为ⅠB亚型 ,亚型间差异小于6 % ;约 4 7%为ⅠA亚型 ,亚型间差异小于 5 3% ;ⅠA与ⅠB亚型间的同源性为 88 7% - 92 3%。结论 中国流行或散发的HAV株可能有基因ⅠA、ⅠB亚型同时存在 ,流行病学相关的病毒株核苷酸序列相同或相近。
