摘要
The fragile rice mutant was isolated from an M2 population of indica variety Shuang Ke Zao (SKZ) treated with γ-rays, and designated as fp1 {fragile plant 1) because of its fragile leaves and culms. To map FP1 locus, an F2 mapping population was derived from a cross between the fp1 and C-bao, a polymorphic japonic variety. The primary mapping result places the FP1 locus in an interval between two molecular markers, microsatellite marker RM16 (3.1 cM proximal to FP1) and STS marker G144a (9.1 cM distal to FP1) in the centromere region of chromosome 3. A CAPS marker C524a was further developed between RM16 and G144a, with 0.4 cM genetic distances to the FP1 locus, providing a practical starting point for constructing a BAC con-tig spanning the FP1 locus and cloning the fp1 gene. Allelism test demonstrated that fpl is allelic to be 1, a fragile rice mutant reported previously.
The fragile rice mutant was isolated from an M-2 population of indica variety Shuang Ke Zao (SKZ) treated with x-rays, and designated as fp1 (fragile plant 1) because of its fragile leaves and culms. To map FP1 locus, an F-2 mapping population was derived from a cross between the fp1 and C-bao, a polymorphic japonic variety. The primary mapping result places the FP1 locus in an interval between two molecular markers, microsatellite marker RM16 (3.1 cM proximal to FP1) and STS marker G144a (9.1 cM distal to FP1) in the centromere region of chromosome 3. A CAPS marker C524a was further developed between RM16 and G144a, with 0.4 cM genetic distances to the FP1 locus, providing a practical starting point for constructing a BAC contig spanning the FP1 locus and cloning the fp1 gene Allelism test demonstrated that fp1 is allelic to bc1, a fragile rice mutant reported previously.
基金
This work was supported by the State Key Basic Research Program (Grant No. 19990116)
the National Natural Science Foundation of China (Grant Nos. 39970409 and 39970871)
the Natural Science Foundation of Zhejiang Province (Grant Nos. 300220 and 300221
