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Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract

Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract
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摘要 When added with cytochrome c, Xenopus laevis egg extract XS-150 can induce exogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, leading to DNA Ladder in electrophoresis. Our results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku. Normally, IXAD exists either in the form of dimmer or in complex with XAD. It was degraded during apoptosis, releasing active XAD. The results of Western assay and cross-inhibition showed that IXAD was likely homologous to DFF45 in structure and function. At the same time, these results also indicated that the pathway in apoptosis was conserved in evolution. When added with cytoch rome c,Xenopus laevis egg extract XS-150 can induce exogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, leading to DNA Ladder in electrophoresis. Our results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku. Normally, IXAD exists either in the form of dimmer or in complex with XAD. It was degraded during apoptosis, releasing active XAD. The results of Western assay and cross-inhibition showed that IXAD was likely homologous to DFF45 in structure and function. At the same time, these results also indicated that the pathway in apoptosis was conserved in evolution.
机构地区 Beijing Univ
出处 《Chinese Science Bulletin》 SCIE EI CAS 2001年第17期1449-1453,共5页
基金 the National Basic Research Project (Grant No. G1999053905).
关键词 XENOPUS LAEVIS APOPTOSIS IXAD XAD DNA LADDER CYTOCHROME c CELL-FREE system. Xenopus laevis apoptosis IXAD XAD DNA Ladder cytochrome c cell-free system
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参考文献19

  • 1Shan Zhu,Zhigang Lu,Yingli Sun,Zhonghe Zhai.Specific degradation of keratin in Xenopus laevis egg extracts undergoing apoptosis[J].Chinese Science Bulletin,2000,45(21):1977-1981. 被引量:1
  • 2Shan Zhu,Zhengfan Jiang,Bo Zhang,Zhigang Lu,Zhonghe Zhai.Identification of a DNase activated in Xenopus egg extracts undergoing apoptosis[J].Chinese Science Bulletin,1998,43(6):522-526. 被引量:4
  • 3Peter G. H. Clarke.Developmental cell death: morphological diversity and multiple mechanisms[J]. Anatomy and Embryology . 1990 (3)
  • 4Z. F. Jiang,S. Zhu,Z. H. Zhai.Induction of mouse nuclear apoptosis in Xenopus egg extracts. Chinese Science . 1998
  • 5Thornberry,N. A.Yuri Lazebnik, Y. Caspases: Enemies within, Science . 1998
  • 6X. S. Liu,H. Zou,C. Slaughter.DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell . 1997
  • 7B. B. Wolf,M. Schuler,F. Echeverri.Caspase-3 is the primary activator of apoptotic DNA fragmentation via DNA fragmentation factor-45/inhibitor of caspase-activated DNase inactivation. Journal of Biological Chemistry . 1999
  • 8C Kayalar,T Ord,MP Testa,LT Zhong,DE Bredesen.Cleavage of actin by interleukin 1 beta-converting enzyme to reverse DNase I inhibition. Proceedings of the National Academy of Sciences of the United States of America . 1996
  • 9Q. Y. Liu,M. Ribecco,S. Pandey.Apoptosis-related functional features of the DNaseI-like family of nucleases. Annals of the New York Academy of Sciences . 1999
  • 10Alnemri ES,Livingston DJ,Nicholson DW,et al.Human ICE/CED-3 protease nomenclature. Cell . 1996

二级参考文献28

  • 1Nagata S.Apoptosis by death factorCell,1997.
  • 2B. Zhang,Y. Chen,Z. Han.The role of keratin filaments during nuclear envelope reassembly in Xenopus egg extracts. FEBS Letters . 1998
  • 3Nagata,S.Apoptosis by death factor. Cell . 1997
  • 4Yaoita,Y,Nakajima,K. et al.Induction of apoptosis and CPP32 expression by thyroid hormone in a myoblastic cell line derived from tadpole tail, J. Biological Chemistry . 1997
  • 5P Li,D Nijhawan,I Budihardjo,SM Srinivasula,M Ahmad,ES Alnemri.Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell . 1997
  • 6A. M. Chinnaiyan,K. O’Rourke,B. R. Lane.Interaction of CED-4 with CED-2 and CED-9: a molecular framework for cell death. Science . 1997
  • 7Liu XS,Kim CN,Yang J,et al.Induction of apoptotic pro-gram in cell-free extracts:requirement for dATP and cyto-chrome C. Cell . 1996
  • 8C Caulin,GS Salvesen,RG Oshima.Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis. The Journal of Cell Biology . 1997
  • 9L. Rao,D. Perez,E. White.Lamin proteolysis facilitates nuclear events during apoptosis. The Journal of Cell Biology . 1996
  • 10V. M. Weaver,C. E. Carson,P. R. Walker.Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes. Journal of Cell Science . 1996

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