摘要
To investigate the clinical significance of monocyte chemotactic protein 1 (MCP 1) produced by endometriotic tissues, the endometriotic tissues were taken from 15 patients with endometriosis. MCP 1 mRNA and MCP 1 protein were determined by dot blot analysis and enzyme linked immunosorbent assay (ELISA) in endometriotic cells cultured with or without interleukin 1β (IL 1β, 2 μg/L), tumor necrosis factor α (TNF α, 20 g/L). After exposure to IL 1β or TNF α, the expression of MCP 1 mRNA in the endometriotic cells (8.635± 0.826, 7.031±0.970, respectively) were significantly higher than that in the control group (4.482±0.435, P <0.05); The expression of MCP 1 protein in IL 1β and TNF α group was 4.52±0.09 μg/L,2.87±0.27 μg/L, respectively, which were significantly higher than 1.74±0.16 μg/L in control ( P <0.01). The results suggested that IL 1β and TNF α could up regulate the expression of MCP 1 in endometriotic cells, which might be related to the development of endometriosis.
To investigate the clinical significance of monocyte chemotactic protein 1 (MCP 1) produced by endometriotic tissues, the endometriotic tissues were taken from 15 patients with endometriosis. MCP 1 mRNA and MCP 1 protein were determined by dot blot analysis and enzyme linked immunosorbent assay (ELISA) in endometriotic cells cultured with or without interleukin 1β (IL 1β, 2 μg/L), tumor necrosis factor α (TNF α, 20 g/L). After exposure to IL 1β or TNF α, the expression of MCP 1 mRNA in the endometriotic cells (8.635± 0.826, 7.031±0.970, respectively) were significantly higher than that in the control group (4.482±0.435, P <0.05); The expression of MCP 1 protein in IL 1β and TNF α group was 4.52±0.09 μg/L,2.87±0.27 μg/L, respectively, which were significantly higher than 1.74±0.16 μg/L in control ( P <0.01). The results suggested that IL 1β and TNF α could up regulate the expression of MCP 1 in endometriotic cells, which might be related to the development of endometriosis.
