不同活化剂对稳定态二氧化氯消毒效果的比较研究

Comparison of different acidic activator in the efficacy of disinfection of stable chlorine dioxide

目的比较盐酸和柠檬酸作为活化剂对稳定态二氧化氯的消毒效果。方法以盐酸和柠 檬酸作活化剂，评价稳定态二氧化氯的消毒效果。结果当消毒剂为４．５ｍｇ·Ｌ－１时，消毒２ｍｉｎ， 对大肠杆菌的杀灭率分别为９９． ９９％及７８． ００％，对金黄色葡萄球菌的杀灭率分别为９９． ９９％及 ９６． ２５％。当消毒剂为９０ｍｇ·Ｌ－１时，１５ ｍｉｎ对细菌芽孢的杀灭率分别为９９．９９％及９７．００％。加 １０％小牛血清的菌悬液要达消毒效果，用盐酸活化只需１８０ ｍｇ·Ｌ－１作用５ｍｉｎ，而柠檬酸则需 ２７０ ｍｇ·Ｌ－１作用１５ ｍｉｎ。以盐酸为活化剂，二氧化氯２００ ｍｇ·Ｌ－１，５ ｍｉｎ即可灭活ＨＢｓＡｇ；而柠檬酸则需４００ ｍｇ·Ｌ－１。结论作为稳定态二氧化氯的活化剂，盐酸明显地优于柠檬酸。

Objective To study which one has a better disinfecting effect on stable chlorine dioxide with hydrochloric-acid or citric-acid as activator. Methods Disinfecting effect was tested with the routine method in accordance with experiment disinfectant. Results The results showed that the efficacy of hydrochloric-acid as an activator excelled that of citric-acid as an activator. The outcomes demonstrated that the stable chlorine dioxide was activated by hydrochloric-acid and cit- ric-acid respectively, the disinfectant containing 4. 5 mg . L-1 available chlorine dioxide could kill 99. 99 % and 78. 00 % Escheritia. coli in 2 min contact time separately and the efficiency for staphylococcus. Aurreus were 99. 99 % and 96. 25 % respectively, the disinfectant solution con- taining 90 mg . L-1 available chlorine dioxide with 15 min contact time could kill 99. 99 % and 97. 00 % bacterial spores respectively when the suspension of the bacterial spores was mixed 10 % calf serum and designed to reach the efficacy of disinfection only to need 15 min with 180 mg . L-1 chlorine dioxide which was activated by hydrochloricacid, but with citric-acid would require 270 mg . L-1 in 15 min. When the stable chlorine dioxide was activated by hydrochloric-acid then the solution will contain 200 mg . L-1 available chlorine dioxide , which can lead to negative conversion for antigenicity of purified, HBsAg within 5 min by SPRLA method. If activated by citric-acid, would demand 400 mg . L-1 available chlorine dioxide. Conclusion As activators of stable chlorine dioxide , hydrochloric-acid was obviously better than citric-acid.