摘要
目的 探讨肿瘤患者外周血T淋巴细胞rDNA转录活性的变化与肿瘤病期或转移的相关性,为临床治疗提供指导。方法 利用rDNA转录活性分析系统及其相关的细胞培养,银染色等方法,对62例恶性肿瘤患者(转移组30例,未转移组32例)的外周血T淋巴细胞rDNA转录活性进行了分析,以核仁积分面积/细胞核积分面积(I.S％)和核仁银染积分光密度/细胞核银染积分光密度(I.O.D％)表示。结果 62例恶性肿瘤患者外周血T淋巴细胞rDNA转录活性的平均水平明显低于正常人群参考值(>7.00),肿瘤或局部广泛侵润或远处转移者则rDNA转录活性进一步下降,转移组与未转移组相比较差异非常显著(t=3.21 P<0.01)。结论外周血T淋巴细胞转录活性从一个方面反映了肿瘤患者的细胞免疫水平。转录活性的下降程度可被认作反映肿瘤病变的敏感指标,对预测肿瘤是否有转移和制定治疗方案具有重要意义。
To discuss the relativity between cancer metastasis and rDNA transcription activity of T-cells, and to providethe principle for the therapy. Methods The rDNA transcription activity of T-cells was detected by a computer-assisted microimage analysis system with silver staining techniques in 62 cases of malignant tumon (metastasis group 30 cases ,non-metastasis group 32 cases). Results The rDNA transcription activity of T-cells was decreased in 62 cases with malignant tumor,statistically significantly lower than that in normal (>7. 00). The results in 30 cases in the metastasis group was decreased obviously, with significant differance as compared with nonmetastasis group (t=3. 21 P<0. 01). Conclusion The rDNA transcription activity of T-cells might be a sensitive and reliable parameter indicating cell immunity level, suggesting that it is valuable in the evaluation of cancer metastasis and in the selection of anti-tumor treatment scheme.
