New procedure for the differential display of mitochondria RNA

A novel approach for the differential display of mitochondria RNA has been described . The total mitochondria RNAs are ligated to a single-strand linker with T4 RNA ligase and then reversely transcripted into the first strands of cDNA. The first strands of cDNA that are ligated to another single-strand linker also with T4 RNA ligase can be directly used as the tem-

A novel approach for the differential display of mitochondria RNA has been described. The total mitochondria RNAs are ligated to a single-strand linker with T4 RNA ligase and then reversely transcripted into the first strands of cDNA. The first strands of cDNA that are ligated to another single-strand linker also with T4 RNA ligase can be directly used as the templates of AFLP. After preamplification, selective amplification and then separating the selectively amplified products on 5% PAGE gel, the display patterns of mtRNA can be generated and the RNAs that represent the differentially expressed mitochondria genes may be identified. This new procedure has been used to analyze the total RNAs that were prepared from the mitochondria of CMS line, maintainer line and F1 hybrid in wild abortive type rice; and one differential band has been detected and then verified.