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Method of transforming resistance gene to carbendazim into Trichoderma sp. 被引量：1

Method of transforming resistance gene to carbendazim into Trichoderma sp.
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摘要 The method of transforming resistance gene to carbendazim into Trichoderma sp. was studied by using genetic engineering technique. The results show that the time of fungicide selection on the transformed Trichoderma sp. may have very important effect on the resistance gene transformation. By this method, the resistance gene to carbendazim was transformed into Trichoderma harzianum, a strain of T. harzianum with resistance to carbendazim was achieved. The transformant could grow on the medium containing 150μg/mL carbendazim. The resistance is stable after 10 times transfer on non-selective medium. The method of transforming resistance gene to carbendazim intoTrichoderma sp. was studied by using genetic engineering technique. The results show that the time of fungicide selection on the transformedTrichoderma sp. may have very important effect on the resistance gene transformation. By this method, the resistance gene to carbendazim was transformed intoTrichoderma harzianum, a strain ofT. harzianum with resistance to carbendazim was achieved. The transformant could grow on the medium containing 150 μg/mL carbendazim. The resistance is stable after 10 times transfer on non-selective medium.
作者 YANG Qian ZHAO XiaoyanCollege of Forestry, Northeast Forestry University, Harbin 150040, China Harbin Veterinary Institute, Harbin 150040, China
出处 《Chinese Science Bulletin》 SCIE EI CAS 1999年第1期65-68,共4页
关键词 GENE of resistance to CARBENDAZIM GENE transformation TRICHODERMA sp. gene of resistance to carbendazim gene transformation Trichoderma sp
分类号 S432.1 [农业科学—植物病理学]
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参考文献11

  • 1Vesa V. Joutsjoki,Tuula K. Torkkeli,K. M. Helena Nevalainen.Transformation of Trichoderma reesei with the Hormoconis resinae glucoamylase P (gamP) gene: production of a heterologous glucoamylase by Trichoderma reesei[J]. Current Genetics . 1993 (3)
  • 2Mark S. Goettel,Raymond J. S. Leger,Srirama Bhairi,M. Katherine Jung,Berl R. Oakley,Donald W. Roberts,Richard C. Staples.Pathogenicity and growth of Metarhizium anisopliae stably transformed to benomyl resistance[J]. Current Genetics . 1990 (2)
  • 3MarkSG,RaymandJSL,SriramaB,etal.PathogenicityandgrowthofMetarhiziumanisopliaestablytransformedtobenomylresistance犤J犦Currentgenetic,1990.
  • 4H Koenraadt,SC Somerville,AL Jones.Characterization of mutations in the beta-tubulin gene of benomyl-resistant field strains of Venturia inaequalis and other plant pathogenic fungi. Phytopathology . 1992
  • 5Sheir-Neiss,C,Lai,MH,Morris,NR.Identification of a gene for B-tubulin in Aspergillus nidulans. Cell . 1978
  • 6NF Neff,JH Thomas,P Grisafi,D Botstein.Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo. Cell . 1983
  • 7Yang Qian.Resistance of Plant Pathogen to Fungicides. . 1995
  • 8Joutsjoki,VV,Torkkeli,TK,Nevalainen,KMH.Transformation of Trichoderma reesei with the Hormoconis resinae glucoamylase P (gamP) gene: production of a heterologous glucoamylase by Trichoderma reesei. Current Genetics . 1993
  • 9Huang, D. F,Bhairi, S,Staples, R. C.Study on transforming Botrytis squamosa with the plasmid (pDH 25) with theresistance gene to Hygromycin B. Biological Engineering Progress . 1990
  • 10Huang, D. F,Zhang, J,Zhang, Z. G. et al.Gene transformation of pathogen of cotton wilt. Progress in Natural Science . 1990

同被引文献10

  • 1CHEN Zhangliang.Application of plant genetic engineering in Agriculture[].High Technique News Letter.1992
  • 2NEFF N F,THOMAS J H,GRISAFI P,et al.Isolation ofthe beta-tubulin gene from yeast and demonstration of its essential function in vivo[].Cell.1983
  • 3MARK S G,RAYMOND J S L,SRIRAMA B,et al.Pathogenicity and growth of metarhizium anisopliae stably transformed to benomyl resistance[].Current Genetics.1990
  • 4LUO K,REN X G,ZHOU B W.Study on the parasitie fungi on sclerotia of Sclerotinia sclerotiorum in rape[].Oil Crops ofChina.1987
  • 5SONG Jinzhu,YANG Qian,WEI Zhigang,et al.A study on biocontrol mechanism ofTrichoderma spp[].With Emphases on Antagonistic Capability Against Sclerotinia Sclerotiorum and Tolerance to Carbendazim.2000
  • 6ELAD Y,CHET I,BOYLE P,et al.Parasitism of Trichoderma spp: On Rhizoctonia solani and Sclerotium rolfsii Scanning electron microscopy and fluorescence microscopy[].Phytopathology.1983
  • 7BENHAMOU N,CHET I.Hyphal interaction between Trichoderma harzianum and Rhizoctonia solani:Ultrastructure and gold chemistry of the mycoparasitic process[].Phytopathology.1993
  • 8YANGQian.ResistanceofPlantPathogentoFungicides[]..1995
  • 9HUANGDafang,BHAIRIS,STAPLESRC. BiologicalEngineeringPrograss . 1990
  • 10HUANGDafang,ZHANGJie,ZHANGZhongge,etal. NaturalScienceProgressNationalKayLaboraryNewsLetter . 1990

引证文献1

Chinese Science Bulletin
1999年 第1期

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