摘要
A DNA fragment about 1.5 kb has been isolated from spleen of adult Chinese swine （?）CR. The DNA fragment encodes immunoglobulin IgG H chain gene. Sequencing analysis showed that the DNA fragment is 1 425 bp long, complete CDS. The C region of the gene has been classified as Subclass lg γ<sub>3</sub>, and is the same as reported by Sun et al., but V region of the present gene is 42 bp less by comparison. The gene has been ligated into expression vector pET-3b （NSEB）（ - ）. A protein about 52 ku has been expressed in E. coil with an expression level of about 21%.
A DNA fragment about 1.5 kb has been isolated from spleen of adult Chinese swine by RT-PCR. The DNA fragment encodes immunoglobulin IgG H chain gene. Sequencing analysis showed that the DNA fragment is 1 425 bp long, complete CDS. The C region of the gene has been classified as Subclass lg γ3, and is the same as reported by Sunet al., but V region of the present gene is 42 bp less by comparison. The gene has been ligated into expression vector PET-3b (NSEB)(-). A protein about 52 ku has been expressed inE. coli with an expression level of about 21 %.
