摘要
Four centromeric segments from B chromosomes (Bs) of rye have been microdis-sected and amplified by linker adapter PCR (LA-PCR). The PCR products ranged from 100 to 2 000 bp. Fluorescence in situ hybridization (FISH) experiment has been carried out using PCR products as the probe, which was labeled by DIG-11-dUTP. The result confirms that these PCR products from Bs centromeric region are homologous with that of A chromosomes (As) in rye. It also proves that Bs are originated from As.
Four centromeric segments from 6 chromosomes (Bs) of rye have been microdissected and amplified by linker adapter PCR (LA-PCR). The PCR products ranged from 100 to 2 000 bp. Fluorescencein situ hybridization (FISH) experiment has been carried out using PCR products as the probe, which was labeled by DIG-11-dUTP. The result confirms that these PCR products from Bs centromeric region are homologous with that of A chromosomes (As) in rye. It also proves that Bs are originated from As.
